Member since: 2021
Organization: Madonna University
Great results, easy to use in the teaching lab!
Application Area: DNA extraction from sperm cells
"I use this product with students in the teaching lab. They can complete their DNA extractions and set up qPCR in the same lab session, which is very useful when they are working with mock crime scene samples. The yield is sufficient for downstream STR amplification and keeps the time required to reach the genotyping stage to a minimum. Highly recommend this product!"
MicroGEM’s forensicGEM™ Sperm lysis kit uses a unique mixture of thermophilic and mesophilic temperature-controlled enzymes to rapidly degrade sperm heads and digest the remaining tissue and nucleases in the sample. At low temperatures, mesophilic cell wall degrading enzymes rapidly degrade sperm heads. The 75° step then activates a thermophilic proteinase that lyses the cells, kills nucleases and strips the DNA of nucleosomes. A final 95° step deactivates the thermophilic proteinase, producing single-stranded DNA perfect for qPCR and STR amplification.
The temperature-controlled DNA extraction process allows for lysis without purification and uses no reducing agents, such as DTT, that inhibit qPCR and STR analysis.