forensicGEM Sperm
forensic GEM Sperm is a rapid kit for extracting DNA from tough sperm heads. Using temperature controls and single-tube process, this kit requires no reducing agents, such as SDS, mercaptoethanol or DTT, that inhibit qPCR and STR analysis, As a result, DNA is preserved, processing time is quick, and opportunities for contamination are minimized making this the perfect approach for differential extractions and y-screening.
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Great results, easy to use in the teaching lab!
DNA extraction from sperm cells
I use this product with students in the teaching lab. They can complete their DNA extractions and set up qPCR in the same lab session, which is very useful when they are working with mock crime scene samples. The yield is sufficient for downstream STR amplification and keeps the time required to reach the genotyping stage to a minimum. Highly recommend this product!
Review Date: 16 Dec 2021 | MicroGEM
MicroGEM’s forensicGEM™ Sperm lysis kit uses a unique mixture of thermophilic and mesophilic temperature-controlled enzymes to rapidly degrade sperm heads and digest the remaining tissue and nucleases in the sample. At low temperatures, mesophilic cell wall degrading enzymes rapidly degrade sperm heads. The 75° step then activates a thermophilic proteinase that lyses the cells, kills nucleases and strips the DNA of nucleosomes. A final 95° step deactivates the thermophilic proteinase, producing single-stranded DNA perfect for qPCR and STR amplification.
The temperature-controlled DNA extraction process allows for lysis without purification and uses no reducing agents, such as DTT, that inhibit qPCR and STR analysis.
- Simplified, single-tube workflow provides STR, PCR, and qPCR-ready DNA in 15 minutes
- Sperm can be extracted in very small volumes and with very few sperm present because there is no loss of DNA to purification steps
- Reduced handling protects the integrity of the sample
- Single-tube processing is compatible with existing thermal cyclers, making it easy to automate for quick DNA extractions.
Brochures
Enabling next-generation molecular techniques & rapid nucleic acid extractions
This brochure demonstrates MicroGEM's aim to advance scientific discovery by developing devices that will allow anyone, whether researcher or layperson, to tackle complex biological problems such as measuring gene expression, detecting specific viruses, or authenticating food attributes or components.
Processing forensic samples using a single-tube approach
Reducing agents, such as DTT, have been used to extract DNA from sperm cells in forensic sexual assault samples for many years. These reducing agents require harsh detergents and denaturants in the lysis buffer to reduce disulfide bonds. The resulting lysate contains reagents and proteins that must be removed via purification steps prior to qPCR and/or STR amplification. In this webinar, Jeff Hickey from MicroGEM, demonstrates how a single-tube protocol can help efficiently process sexual assault samples, including Y screening and differential extractions.
