Member since: 2022
Organization: YENEPOYA RESEARCH CENTRE
A standard sterile medium for isolating human immune cells for in vitro studies.
Application Area: PBMC isolation
"Ready to use density gradient medium for high yield isolation of human, mouse, and rat low-density mononuclear cells from peripheral blood. It allows PBMCs to be isolated from whole blood upon centrifugation."
Member since: 2019
Organization: West Virginia University
Efficient separation of granulocytes and PBMCs!
Application Area:Separation of PBMCs from erythrocytes and granulocytes
"The Ficoll-Paque gradient has been wonderful for separating PBMCs from granulocytes and red blood cells. We use a specific cell type that isn't as characterized as others and has some similarities to granulocytes, without the denseness, so using this Ficoll helps to make sure we separate as much of the granulocytes as possible from the cell type we want in the PBMCs. We place the Ficoll at the bottom of our tubes after the blood is already in, so it's a bit of an art to make sure the Ficoll stays separated from the blood, but once you get it, it separates wonderfully each time."
Ficoll-Paque Plus is a recognized standard in laboratories worldwide for the isolation of human lymphocytes for in vitro studies.
Ficoll-Paque PLUS is a ready to use, sterile medium for isolation of lymphocytes in high yield from peripheral blood using a simple and rapid centrifugation procedure. It maintains the viability and a representative distribution of B and T lymphocytes.
Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.
Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.
Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.
Ficoll-Paque PREMIUM 1.084
Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.
Ficoll-Paque PREMIUM 1.073
Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).
Manufacturer Cytiva4.7 / 5.0 | 2 reviews