Member since: 2020
Organization: Erasmus MC
Great kit. Very easy to use!
Application Area: 16S quantification
"I really love this product. It's extremely easy to use. Standards are already diluted, providing consistency between runs and the master mix contains all the components necessary. Product is able to detect very low amount of 16S in samples. Only thing it could improve is the throughput, it would be nice to be able to use it in a 384 well format. Kit is designed for 96-wells."
Member since: 2019
Organization: Centre for Cardiovascular Surgery and Transplantation, Brno
Great way to control DNA amount in a sample before shotgun sequencing.
Application Area:Quality control before NGS
"Product is easy to use, the reaction is robust enough to measure bacterial cells in low DNA input samples. Together with Femto Human DNA kit, it provides the possibility of differentiating bacterial and human DNA in a sample before shotgun sequencing in the case of clinical samples with low bacterial DNA input."
The Femto™ Bacterial DNA Quantification Kit can detect and quantify bacterial DNA with high specificity and sensitivity. Bacterial DNA can be reliably quantified in a background of non-bacterial DNA such as fungal, animal, plant DNA, etc. This is essential for downstream applications that require accurate DNA input amounts such as quantifying bacteria DNA template for Next-generation sequencing library preparation and metagenomic analysis. With the Femto™ Bacterial DNA Quantification Kit, dependably quantify as little as 20 fg of bacterial DNA from 1 µl of purified biological liquids, bacterial cultures, or environmental DNA samples.