Fast-Link™ DNA Ligation Kit by Lucigen Corporation

Manufacturer Lucigen Corporation
4.3
/
5.0
  |  1 reviews
Transform into E. coli without clean-up or desalting
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Fast-Link™ DNA Ligation Kit by Lucigen Corporation product image
cohesive end ligation

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Average Rating: 4.3
1 Scientist has reviewed this product

5 out of 5
Ease of use
3 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Advanced Reviewer
  • Member since: 2021

  • Organization: Marine Biological Laboratory



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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Fast-Link for sure.
Rating: 4.3

  • Application Area: Molecular cloning

"Fast-Link ligase works like a charm, easy fast and sure, what else do you need for your routine cloning work?"

Review date: 22 Mar 2021 | Fast-Link™ DNA Ligation Kit

Applications

  • Blunt-end and TA cloning of PCR products
  • Ligation of next-gen sequencing adaptors to blunt-ended DNA
  • Genomic DNA cloning and subcloning
  • BAC/fosmid library construction
  • cDNA cloning
  • Linker ligation

The Fast-Link™ DNA Ligation Kit uses a high-quality ligase (Fast-Link T4 DNA Ligase), that was cloned at Epicentre and formulated to provide extremely rapid, high-efficiency DNA ligation. Cohesive-end ligations can be performed in 5 minutes at room temperature. In contrast to other ligases, it is not necessary to desalt Fast-Link ligation reactions prior to the transformation of electrocompetent or chemically competent cells. The Fast-Link Kit can be used for routine and high-throughput DNA cloning.

Benefits

  • Cohesive-end ligations in 5 minutes at room temperature
  • Blunt-end ligations in 15 minutes at room temperature
  • Ligation of PCR product with A-overhangs in 1 hour at 16°C
  • High ligation efficiency
  • Desalting of ligation products is not needed prior to the transformation of electrocompetent or chemically competent cells
  • BAC ligation with inserts >100 kb can be performed in 4 hours, instead of overnight reactions as with other ligases

Product Overview

Fast-Link™ DNA Ligation Kit by Lucigen Corporation product image

Fast-Link™ DNA Ligation Kit

Manufacturer Lucigen Corporation

4.3 / 5.0 | 1 reviews