EZ DNA Methylation-Direct™ Kit by Zymo Research

Manufacturer Zymo Research  |   Model: D5020
4.8
/
5.0
  |  20 reviews
The EZ DNA Methylation-Direct™ Kit containis reagents necessary for complete bisulfite conversion of DNA directly from blood, soft tissue, cells, FFPE samples, and LCM samples.


EZ DNA Methylation-Direct™ Kit by Zymo Research product image
EZ DNA Methylation-Direct Bisulfite Conversion Kit
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Average Rating: 4.8
20 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2022

  • Organization: Tufts University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellen product for epigenetic analysis
Rating: 5.0

  • Application Area: Mice tumor tissue

"Excellent product with fewer steps to convert DNA from tissue. High recovery of DNA converted to use for epigenetics analysis."

Review date: 21 May 2022 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Federal University of Ceará (UFC)



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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I've been using this kit for 4 years and I've never had any problems with results.
Rating: 4.7

  • Application Area:Targeted bisulfite sequencing analyzes

"I have used several batches of this kit. The conversion efficiency of unmethylated cytosines is adequate for the bisulfite sequencing test. The kit has two types of use in the incubation part, I always recommend using 16X cycling over night for better performance. It is an easy handling kit and the protocol contains several performance improvement tips. The amplification results with the DNA modified by the kit are very good, but it is always important to do a good standardization of primers beforehand."

Review date: 30 Mar 2022 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good choice for the bisulfite conversion of LCM samples
Rating: 5.0

  • Application Area:Bisulfite conversion of laser capture microdissected samples

"I have been using this kit for years now routinely for the bisulfite conversion of the challenging laser capture microdissected (LCM) tissue samples. It has the advantage to be able to skip DNA isolation step, so bisulfite conversion step can be performed with minimal input material loss with a high conversion rate"

Review date: 25 Jan 2022 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: King George's Medical University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Very easy to use and reliable results
Rating: 5.0

  • Application Area:Methylation status of selected genes in OSCC tissue samples

"The EZ methylation kit is easy to use and very cost effective. It produces results which are reproducible."

Review date: 18 Dec 2021 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Banaras Hindu University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Amazing results in every batch of this product.
Rating: 5.0

  • Application Area:Mouse embryonic development tissue

"It was an amazing experience to use this kit. The most important point is 100% results. I have got success in every reaction and all the downstream experiment results confirm the efficiency of the kit. I am pretty sure about its high applicability and my future use. I have no words for Zymo technical support. They provide endless support. I am so happy with the reproducibility of the product. It has helped me in maintaining my image in front of my supervisor. After thesis submission, I am planning to write some projects based on Zymo products. I have used this product on both animal and plant samples and the results are amazing. We are 7 students in the lab and almost all have used this product."

Review date: 16 Nov 2021 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: UW Medicine Health Sciences



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Our number one kit of choice for all our bisulfite sequencing samples.
Rating: 5.0

  • Application Area:Bisulfite conversion of DNA for whole genome bisulfite sequencing

"This product is easy to use and gives high quality results in very little time."

Review date: 28 Jul 2021 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Semmelweis University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Clear protocol, reliable product!
Rating: 5.0

  • Application Area:Bisulfite conversion of cell-free DNA molecules for methylation analyses

"We are absolutely satisfied with this product. The protocol is easy to follow, and very user-friendly. The control tests show that the measurements are reproducible, and the results are completely reliable. We use this kit almost every week and we haven’t been disappointed in it yet. Thanks."

Review date: 28 Jul 2021 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Baylor College of Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results.
Rating: 5.0

  • Application Area:Methylation analysis in different mice tissues

"My control had a 99.8% conversion rate which is impressive."

Review date: 09 Jun 2021 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Tufts University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Product of high quality.
Rating: 4.3

  • Application Area:DNA methylation tissues/embryos

"When I was looking for a advice for DNA methylation, the customer service was very efficient and helped me to find the correct product for my research. I tried the kit they suggested and now I have ordered it again. It's easy to use it."

Review date: 05 Jun 2020 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Kyungpook National University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Good results.
Rating: 4.0

  • Application Area:DNA methylation

"It was easy to use and also got results from it. Good enough."

Review date: 05 Jun 2020 | EZ DNA Methylation-Direct™ Kit


  • Status:

    Reviewer

  • Member since: 2019

  • Organization: USC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results!
Rating: 5.0

  • Application Area:Circulating tumor cells

"This product produces high quality, reproducible results, which is highly important in this area of research. I highly recommend it and it is reliable!"

Review date: 27 Nov 2019 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Victoria University of Wellington



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Inconsistent results.
Rating: 4.7

  • Application Area:Analyse DNA methylation in animal tissue

"Very easy to use, but unreliable results. Good price."

Review date: 05 Jun 2019 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Mayo Clinic



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Great results!
Rating: 4.7

  • Application Area:Analyze methylation marks in tissue

"Excellent product, easy to use, was able to complete the task I had in hand. I will definitely purchase again and recommend to colleagues."

Review date: 05 Jun 2019 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Geisel School of Medicine at Dartmouth



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great product with excellent ease of use.
Rating: 4.3

  • Application Area:DNA methylation in Epigenome-wide association studies

"The field leader in bisulfite conversion for experiments assaying DNA methylation in human and murine tissues."

Review date: 29 Jul 2016 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: GENETIC RESEARCH INST. OF THE DESERT



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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It's a great kit.
Rating: 4.0

  • Application Area:DNA methylation

"This kit is user friendly and gives very good results. The protocol is very clear and any individual with no knowledge can follow directions and use this kit."

Review date: 28 Jul 2016 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UNESP - Sao Paulo State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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The best cost-benefit ratio
Rating: 5.0

  • Application Area:DNA methylation analysis

"Efficient conversion of unmethylated cytosines for downstream DNA methylation analysis, including pyrosequencing, sanger sequencing as well as PCR-based approaches. Protocol based on few steps easy-to-use."

Review date: 28 Jul 2016 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Advanced Reviewer

  • Member since: 2016

  • Organization: McGill



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Very accurate results as we verified using other methods.
Rating: 5.0

  • Application Area:Analyze sample from cell cultures

"This is an excellent kit for measuring global DNA methylation. Whole procedure is simple even the novice with limited experience in molecular biology could use it and have reproducible results. This kit is cost and time-effective compared to other methods for measuring DNA methylation like HPLC."

Review date: 25 Jul 2016 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Fertility Labs of Colorado



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great for small template, easy to use!
Rating: 5.0

  • Application Area:Methylation analysis

"This kit is a great product and easy to use. We work with very small template and had concerns regarding DNA loss after bisulfite conversion. This has not been an issue however and we've had great success with our experiments. The protocol is easy to follow and requires little hands-on time. It is by far the best kit for the price!"

Review date: 13 Jul 2016 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Washington



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Good product
Rating: 4.3

  • Application Area:bisulfite conversion of DNA

"Obtain reproducible results, easy kit. It is good value for the money. Can use on a variety of sample types."

Review date: 12 Jul 2016 | EZ DNA Methylation-Direct™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Illinois



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Made analysis great for a first time researcher
Rating: 5.0

  • Application Area:Genomic DNA methylation assessment

"Great salespeople that I encountered at Experimental Biology. Explained how the product worked and were very helpful."

Review date: 12 Jul 2016 | EZ DNA Methylation-Direct™ Kit

The EZ DNA Methylation-Direct™ Kit is a further refinement of our popular EZ DNA Methylation™ and EZ DNA Methylation-Gold™ kits.

These products feature reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of these kits make it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Like the EZ DNA Methylation-Gold™ kits, DNA denaturation and bisulfite conversion processes are combined into a single step. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.

Product Overview

EZ DNA Methylation-Direct™ Kit by Zymo Research product image

EZ DNA Methylation-Direct™ Kit

Manufacturer Zymo Research

4.8 / 5.0 | 20 reviews