EZ-96 DNA Methylation Kit by Zymo Research
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Status:
Reviewer
Member since: 2019
Organization: DKFZ-Heidelberg
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- Ease of use
- After sales service
- Value for money
Zymo Kit D5004 for chip technology.
Application Area: Illumina epic chips
"We use Zymo D5004 Kit to Convert our DNA-samples for Illumina chip technology. We have used it for more than 10 years. We are very pleased with it."
Review date: 27 Nov 2019 | EZ-96 DNA Methylation Kit
The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA.
Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulfonation technology eliminates otherwise cumbersome precipitations.
The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Key features include:
- Streamlined, proven procedure for bisulfite conversion of DNA.
- Desulphonation and recovery of bisulfite-treated DNA with a 96-well spin-column plate.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
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