Status:
ReviewerMember since: 2019
Organization: DKFZ-Heidelberg
Zymo Kit D5004 for chip technology.
Application Area: Illumina epic chips
"We use Zymo D5004 Kit to Convert our DNA-samples for Illumina chip technology. We have used it for more than 10 years. We are very pleased with it."
The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA.
Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulfonation technology eliminates otherwise cumbersome precipitations.
The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Key features include: