EpiMark® Hot Start Taq DNA Polymerase by New England Biolabs Inc.

EpiMark Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The advanced aptamer-based hot-start activity coupled with the supplied optimized reaction buffer makes the EpiMark Hot Start Taq DNA Polymerase an excellent choice for use on bisulfite-converted DNA.

Taq DNA polymerase possesses a 5´- 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

EpiMark Hot Start Taq DNA Polymerase is supplied with 5X EpiMark Hot Start Taq Reaction Buffer, providing robust amplification for bisulfite-converted DNA and AT-rich amplicons.

Taq DNA Polymerase optimized for epigenetics analysis

• Ideal for use on bisulfite-converted DNA and AT-rich templates

• Specially formulated reaction buffer system

• Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup

Applications:

• Bisulfite Sequencing

• Hot Start PCR

• Specialty PCR

• Routine PCR

• PCR