Member since: 2019
Organization: University of Utah
Great results in PCR with CG-rich DNA.
Application Area: Polymerase Chain Reaction (PCR)
"Very effective in our PCR for bisulfite converted DNA and also CG-rich DNA."
EpiMark Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The advanced aptamer-based hot-start activity coupled with the supplied optimized reaction buffer makes the EpiMark Hot Start Taq DNA Polymerase an excellent choice for use on bisulfite-converted DNA.
Taq DNA polymerase possesses a 5´- 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).
EpiMark Hot Start Taq DNA Polymerase is supplied with 5X EpiMark Hot Start Taq Reaction Buffer, providing robust amplification for bisulfite-converted DNA and AT-rich amplicons.
Taq DNA Polymerase optimized for epigenetics analysis
Ideal for use on bisulfite-converted DNA and AT-rich templates
Specially formulated reaction buffer system
Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup
Hot Start PCR