DNA methylation was the first epigenetic modification identified and has been intensively studied for more than half a century. By now it is clear that Dnmt1, the major eukaryotic DNA methyltransferase, faithfully maintains genome-wide methylation patterns and plays an essential role in the epigenetic network controlling gene expression and genome stability during development. However, the molecular mechanisms that ultimately control DNA methylation still remain elusive. Our epigenetic Toolbox contains innovative tools to make new discoveries:
The high quality Dnmt1-binding protein is coupled to agarose beads for biochemical analysis of Dnmt1 proteins and their interacting partners.
Use Dnmt1-Trap® for:
• (one-step) Pulldowns/ Immunoprecipitations
• Mass spectroscopy
• Enzyme activity measurements
• ChIP analysis
The Dnmt1-Chomobody® comprises the antigen binding domain (VHH) derived from heavy chain antibodies of Alpaca genetically fused to TagGFP2 or TagRPF (Evrogen). This Chromobody® allows you to visualize endogenous Dnmt1 in real time in live cells.
• Transfect these fluorescently labeled nano-probes into your cells of interest
• Dnmt1-Chromobody® is the perfect cellular high content screening tool for inhibitors/activators of Dnmt1.
Conventional Dnmt1 antibody (2E8)
To complement the Alpaca Antibody Advantage based products, that recognize native protein, we also offer a rat monoclonal for applications where the denatured protein needs to be detected: Western blot and IF applications.