Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System by Wyatt Technology Corp.

Manufacturer Wyatt Technology Corp.  |   Model: WECLAQ / WECLMF / WE  |  Available Worldwide
4.6
/
5.0
  |  9 reviews
Versatile separations of polymers, proteins, colloids, and nanoparticles.


Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System by Wyatt Technology Corp. product image
Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System
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Average Rating: 4.6
9 Scientists have reviewed this product

4 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2020

  • Organization: STEMCELL Technologies



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great instrument that has significantly improved our research capabilities!
Rating: 5.0

  • Application Area: Characterization of antibodies, nanoparticles and extracellular vesicles

"We've had the Eclipse AF4 flow field flow fractionation system in our research and development lab for over a year now. The system itself is very easy to use, especially if you have a basic understanding of HPLC and/or SEC. We have been able to obtain successful separation of samples containing both digested and undigested antibodies, and isolation of nanoparticles from multiple supernatant components. Wyatt customer service is great - every time we have run into an issue, we have gotten a response from support in less than 24 hours and they have always been able to solve our problem. The instrument is very expensive, but it is highly versatile and has become a key piece of instrumentation in our lab."

Review date: 01 Jun 2020 | Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Georgia Institute of Technology



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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It is our view that the Wyatt Eclipse AF4 will replace GPC as the industry standard.
Rating: 5.0

  • Application Area:Molar mass, size and shape information for water soluble polymers, proteins and particles

"Field flow fractionation is a very difficult technique to master but the Wyatt Eclipse AF4 unit with included Scout predictive software, in addition to their 24/7 customer service, made method development and analyte characterization very achievable and enjoyable. Using one single instrument we have managed to separate and characterize polyelectrolytes with small radii from several nm up to hundreds of nm long rod like cellulose nanocrystals. The versatility and capability of this instrument is incredible and Wyatt's field reps must be credited for helping us realize the full potential of what AF4 can do."

Review date: 27 May 2020 | Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Eawag



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Wyatt Europe is the best partner for all service. Interesting webinars and exceptional customer service.
Rating: 4.3

  • Application Area:Analyzing NP-particle interactions with organic colloids in environmental systems

"With Wyatt Europe, I have a really good partner in after sales and application development/method development. The system is very flexible and with the service contract I always can count on immediate remote-help. Although, I am not very happy with the dictated combination of hardware and software developments meaning that I am somewhat dependend on expensive software-changes for optimal use of the hardware, and respective bug-fixes. As a university research institute we don't have the money to change working hardware, like in industrial settings. Wyatt US only provides online help if the instrumentation is not older than 10years, which means the possibility to satisfy customers like us is only possible by making a lot of effort. The free webinars offered to us during COVID19 were more than helpful, including the user seminars in the last weeks."

Review date: 27 May 2020 | Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Zentriforce Pharma



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Nice and easy to use AF4 system.
Rating: 5.0

  • Application Area:ATMP, Peptides and Antibodies

"Nice and easy to use AF4 system. For an AF4 system it is amazingly robust and can be applied to a wide range of applications. The option to use a frit-inlet channel is useful to test for focus stress induced changes of your material."

Review date: 17 Apr 2020 | Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: IBT-VAST



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great system, must-have for high IF paper.
Rating: 4.0

  • Application Area:Exosome fractionation

"The system works well for the purpose of study which is separation of exosome based on size. Although setup and optimization of instrument are quite complicated, the support staff are well-trained and professional. I usually get replies to emails email within 1-3 working days."

Review date: 24 Dec 2019 | Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Seqirus Inc.



  • Ease of use
    2 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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AF4 has great potential to fractionationate without the adverse effects of LC/SEC.
Rating: 4.0

  • Application Area:Influenza vaccines, proteins, lipid nanoparticles,

"The instrument is designed well for ease of use in batch or being modular for online mode. However, the software needs to be well integrated so that it is a more cohesive software package where all of its capabilities are centralized into one package. As a result the software has a steep learning curve. Also, the AF4 channel design can definitly be improved with torqued latches so that assembly can be done more simplistically by flipping latches instead of manually by hand with a torque wrench."

Review date: 28 Aug 2019 | Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Leidos Biomedical Inc.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use
Rating: 5.0

  • Application Area:Nanoparticle characterization

"We used Wyatt Eclipse AF4 coupled with online MALS and DLS for liposome, nanoparticle, and other formulation characterization. It is easy to use, and is able to produce high quality results."

Review date: 29 Jan 2018 | Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: 3M (Pace Analytical)



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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This instrument has proved invaluable for the analysis of very difficult samples.
Rating: 4.3

  • Application Area:polymers, nanoparticles

"This system is able to separate ultra high molecular weight polymers as well as highly branched polymers. Additionally, we are able to determine the radius of different sized nanoparticles and colloids."

Review date: 20 Jul 2017 | Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Lund University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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I had good results
Rating: 5.0

  • Application Area:macromolecules

"Asymmetrical flow field-flow fractionation when connected to suitable detector such as multi-angle light scattering (MALS) has shown to be a great technique for the analysis molar mass and root mean square of many food macromolecules. The results are reproducible and with good quality"

Review date: 24 Jun 2016 | Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System

The Eclipse® AF4 solves the most difficult separation, fractionation and sizing tasks in the macromolecular and colloidal arenas. Because it has no stationary phase, the advantage of asymmetric-flow field-flow fractionation (AF4) over traditional SEC column chromatography is the ability to separate both soluble and colloidal components, no shear and little to no adverse surface interactions. Integrates with industry-leading HPLC modules. 

The Eclipse is most often used with a DAWN® multi-angle light scattering (MALS) detector and ASTRA® software for FFF-MALS-DLS analysis of molar mass, size and conformation distributions.

Features: 

  • Separation range: 1 – 10,000 nm
  • Solvent compatibility: 2 models - aqueous (biocompatible) and organic solvents
  • Supported HPLC modules: The Eclipse AF4 integrates with Agilent, Thermo and Shimadzu pumps and autosamplers as well as degassers, online detectors and mass spectrometers
  • Supported separation channels: 2 sizes of standard-injection analytical AF4; frit-inlet channel for sticky or aggregation-prone samples; Mobility® electrical/AF4 module for zeta-potential analysis; and semi-prep channel for mg-scale separations
  • Software: AF4 and HPLC modules may be controlled from native HPLC software packages with plug-ins, or from the VISION® chromatography software suite which includes SCOUT DPS® for virtual method development and size analysis, and controls ASTRA software for MALS/DLS analysis 

Applications: 

  • High-resolution molar mass and size distributions of proteins, soluble and insoluble aggregates, natural and synthetic polymers, polymersomes, exosomes, liposomes, viruses, virus-like particles, metallic nanoparticles, quantum dots, drug-delivery and diagnostic nanoparticles, emulsions and many more
  • Analysis of fragile macromolecules or particles that are sheared on a size-exclusion column, sticky proteins that adhere to the stationary phase, or large, heavily-branched polymers that elute abnormally from GPC columns
  • Separation and characterization of trace nanoparticles from environmental matrices such as soil or seawater
  • Detailed size-zeta potential distributions of nanoparticles
  • Semi-preparative separations
  • AF4 is recognized by the FDA and other regulatory agencies as an orthogonal technique to size-exclusion chromatography for analysis of biopharmaceutical aggregation.

Product Overview

Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System by Wyatt Technology Corp. product image

Eclipse AF4 Asymmetric-Flow Field-Flow Fractionation System

Manufacturer Wyatt Technology Corp.  |  Available Worldwide

4.6 / 5.0 | 9 reviews