Member since: 2016
Organization: University of Michigan
Achieved half a way of the experiment.
Application Area: Isolation of neutrophils
"The kit is provided by the protocol, which works nicely. The problem is that it is capable of removing only thrombocytes and platelet, not other WBCs like monocytes and leucocytes. I was able to get only 50% neutrophils. The experiment requires more than 90% of the neutrophils."
The EasySep™ Mouse Neutrophil Enrichment Kit is designed to isolate neutrophils from single-cell suspensions of bone marrow or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-neutrophils. Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.