DNA/RNA Shield™ by Zymo Research

"The DNA/RNA Shield allows you to store a sample for microbiome analysis, keeping its composition unchanged (it freezes an image at the time of sample collection!). It is a product that is simple to handle and much cheaper than other options available on the market. We used this product in conjunction with extraction using ZymoBIOMICS and it allowed us to obtain a high-quality microbiome analysis, the results of which were published in Frontiers in Immunology."

"I used this media to collect saliva specimens which were then put through an automated MagMax extraction. DNA was quantified and while we didn't see as high of a yield with saliva as we saw with swabs, this solution definitely enhanced the quality of the saliva specimens and their stability."

"It has preserved the DNA as well as RNA in good condition. We are using nasal polyp tissues of the allergic patients and the DNA/RNA shield was very helpful in studying the nasal microbiome."

"DNA/RNA shield has been great for preserving my coral tissue samples, and I have gotten high yields of DNA and RNA from my coral tissue extractions after using this product! I highly recommend it to coral biology researchers."

"We are using this product to store blood samples for a long time and it helps us to obtain quality and higher amount of DNA/RNA yields in blood samples after a longer (2 years) of storage time."

"DNA/RNA Shield is easy to use, allows for sample flexibility, and stabilizes our samples well for downstream sequencing runs."

"This product was the easiest way to ensure my scat samples from an endangered primate was rendered non-infectious quickly and easily, so that we could extract the primate DNA with confidence."

"Shield is easy to use, and allows removal of high containment samples without live virus after 1 hour at room temperature. The integrity of the RNA is maintained for qRTPCR, and it is easily used with Qiagen's RNA prep kit."

"It's very easy to use, preserves my samples well and relatively cost-effective. This is so important for me as a researcher from a developing country with limited access to funds."

"In general, I love Zymo reagents and kits. Their tech support is wonderful and helpful. DNA/RNA shield in particular is amazing because I can store my samples in the field in it and not have to worry about pathogen inactivation and I can just go straight into the extraction kit when back in the lab."

"Did its job well, no issues at all."

"A reliable product that keeps our DNA protected in clinical and research samples. This product has extended the shelf-life of many of our samples."

"I got the 2x solution and used it routinely for months over the course of a project. Just mix and store, no muss - no fuss."

"This product was great and easy to use for safely storing nucleic acid samples from bacteria."

"With this product we are able to collect and store samples without needing liquid nitrogen and space in the -80 freezer while maintaining the microbial community composition."

"We're doing a study in which we are subjecting tissue samples to a full next-generation sequencing, both DNA and RNA. All I have to do is sterile pipette this solution into sterile 1.5mL tubes - the samples get dropped right in and their DNA and RNA is preserved, even at room temp. If all you're interested in in DNA and RNA, it makes the pipeline much easier."

"In my experience this buffer helps us to process the samples of patients' COVID19 without risk. Is a little expensive but the service is excellent "

"Product works well to preserve nucleic acids for downstream sequencing and other molecular biology applications."

"I have used several types of sample collection kits. By far Zymo's DNA/RNA Shield is the best. My samples are protected from degradation and reduce sample shipping costs. I have found that the reagent is compatible with every extraction, PCR, and NGS sequencing protocol I have used. All in all, it is my go to choice for sample collection."

"It is a very easy to use product that allows us to preserve samples without losing integrity. Simply cover solid samples and add multiple volumes of liquid to extract nucleic acids when it suits you best. They even keep at room temperature."

"For a long time we have been travelling with cooler boxes, which is not only tiresome but does not give reproducible end results, after trying the DNA/RNA shield, this has completely changed how we see our work. The results are great, no more cooler boxes, DNA/RNA shield preserves our intended nucleic acid for more than three days worry free. The local distributors from Zymo assured before and after their support. I completely recommend this for scientists working in the field."

"This reagent is very helpful for preserving viral RNA in our workflow! Thank you."

"It was great. High yield and good quality RNA. Transcriptomic libraries are perfect."

"This product increased my RNA quality and yield after use! Definetely suggest using this with DNA and RNA."

"The DNA storage was highly effective. The results from NGS were reproducible."

"This solution is fantastic for stabilizing DNA and RNA at room temperature, which is great for sample shipping and taking your time in sample preparation steps. Extra bonus is meeting the CDC inactivating requirements, which allows easier shipping of samples from COVID-19 patients. The Zymo team are also worth so much more than just the product, their tech support are actual people who work in the lab, so they will know the details and can easily ask more technical questions to the person who actually designed the product. I’ve talked to the creator of this solution a few times (Luigi), such as when we wanted to do metabolomics, and he is always clear and helpful. They also optimize products for the user, I often find when benchmarking kits that Qiagen ones take at least twice as long to perform. Would suggest Zymo for all your genetics needs."

"The product preserved my samples until they were ready to be analyzed. This was a game changer because I didn’t need to store them in a freezer, and I didn’t need to ship them in a special container which also saved me money."

"Good and easy to use. The samples resist a lot when in the product."

"I used DNA/RNA shield to maintain the cold-chain. My samples, as diverse as bacteria from biofilm, fecal samples or WWTP bacteria can be stored before extraction. The samples can be kept at room temperature so I can plan my extractions for the most convenient time. The product is easily removed before extraction."

"The DNA/RNA Shield was highly efficient to protect the RNA samples I used in my experiments. The protocol was also very easy to perform. It was definitely worth buying this and I recommend this product highly for other researchers."

"We received a trial kit with 50 tubes. They were used to collect blood samples in South America, where we are currently doing research on malaria and dengue. We have extracted RNA from the tubes with no problem. The RNA yield is superior and of better quality than using filter paper. The best is that researchers in the field do not need a refrigeration system to keep the tubes after blood has been obtained."

"DNA/RNA shield was a very effective method to isolate RNA and DNA from various mouse tissues. The purity of RNA and DNA was very high and we received a high yield from the small amount of tissue we used."

"We are working to collect environmental water samples for microbial community analysis. Our first attempt had us collecting the water samples, trying to keep them cold until we got back to the lab followed by immediate DNA extraction. The day was long, stressful, and unsuccessful. Enter DNA/RNA Shield. This item is designed for being in the field. It is stable at ambient temps, does not require any fancy or complicated procedures, neutralizes pathogens, and got us great results. We simply added the water sample to the DNA/RNA Shield and we were done. We also used Zymo's ZymoBiomics service for this project. Once we had the samples, all we had to do was pack them up and send them in. It was too easy. We got back amazing results from our samples that we could not have gotten in-house. As I was sharing this with a colleague who was working on blood for genomic analysis, she accused me of witchcraft. She is now using this product as well and getting great, easy results."

"The product is easy to use and the instructions are clear. The seller contacted us right after we bought for the first time. The price is affordable. The quality of the product is good and it can be sent to other places without the need of storing it at 4 degrees C. We didn't have any problems with the results and the DNA was as expected as in a fresh sample."

"DNA/RNA shield was very effective when i used it to store microbial cells. It was effective in stabilizing the DNA of the cell untill when used for analysis. It gave the required result. I could easily transport sample without fear of loss of nucleic acid stability. The DNA from my sample was also easily recovered when I then wanted to use it. The product is very easy and simple to use, there is not much protocol to follow. Anyone working with nucleic acids in samples can use DNA/RNA shield to store and maintain the nucleic acid of his/her samples during analysis and for future analysis. The product deserves a 5 star rating in all categories of rating. There's no long protocol during use. Although, I am not the purchaser, the product is cost-effective, the satisfaction derived from use of the product is commendable."

"We have been using RNAlater for whole blood nucleic acids stabilization. However, samples have to be processed prior to extraction (i.e. separate and remove RNAlater). This additional step is not easy especially when handling 100s of samples. With DNA/RNA Shield™, stabilized blood samples can be directly applied to the purification step. We found this really advantageous over the other stabilization buffers in the market."

"The Shield product works well on difficult to homogenize samples. We can increase the time and speed of our Beadmill to thoroughly homogenize while preserving DNA intergrity. Previously we could get good RNA and good DNA, but poor yields. Now we can get good RNA, DNA and good yields."

"Great product, easy to use and is worth the investment for downstream applications."

"DNA/RNA Shield™ is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!"