DNA-free Mastermix 16S Complete
Universal 16S rDNA real-time PCR assay for sensitive detection of bacterial DNA
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DNA-free Mastermix 16S Complete is an eubacterial master mix with strong amplification activity and accuracy including universal 16S primers covering the V3/V4 variable region of the 16S rRNA gene. This allows a highly sensitive detection of femtogram amounts of bacterial DNA. Mastermix 16S Complete includes fluorescent dye for real-time PCR as well as gel loading solution and DNA size marker for standard PCR.
Benefits:
- Ultra-sensitive detection of bacterial DNA
- Guaranteed microbial DNA-free
- Highly active Taq DNA polymerase
- High amplification activity for 40 cycles
- Validated for standard PCR and Real-time PCR
Ultra-pure Mastermix 16S Complete combined with Molzym's unique DNA isolation technology, MolYsis™, will lead to a significant increase in the PCR sensitivity.
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Selective Enrichment of Bacterial and Fungal DNA
MolYsis™ is a tool for the removal of human DNA and enrichment of bacteria and fungi from liquid samples. Technically, in a short series of steps, MolYsis™ effects the selective lysis of blood cells and the quantitative degradation (>99%) of released human DNA. After enrichment from the lysate by centrifugation, microorganisms are treated with a reagent degrading cell walls of Grampositive and Gram-negative bacteria and fungi. The broad range lysis potential has been shown in clinical evaluations, including over 200 genera of bacteria (86 Grampositives, 120 Gram-negatives) and 65 genera of fungi.
Hot MolTaq 16S/18S: DNA-Free PCR Reagents
A common drawback of PCR assays for bacterial and fungal sequences is the contamination of amplification reagents by microbial DNA. As a consequence, false-positive results may arise often also associated with a loss of analytical sensitivity. Molzym‘s Mastermix 16S/18S products are guaranteed free of contaminating bacterial and fungal DNA. This provides accurate results also at low target loads.
