DNA-free Mastermix 16S/18S Basic
Ultra-clean PCR reagents for use with custom primers
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DNA-free Mastermix 16S/18S Basic is an ultra-clean master mix for use with custom primers or probes and is especially suited to amplify bacterial or fungal DNA. The included Taq DNA polymerase is highly active and allows sensitive detection of femtogram amounts of microbial DNA with Real-time or standard PCR.
Benefits:
- Ultra-sensitive detection of target DNA
- Guaranteed microbial DNA-free
- Highly active Taq DNA polymerase
- For use with custom primers or probes
- High amplification activity for 40 cycles
- Validated for standard PCR and Real-time PCR
Ultra-pure Mastermix 16S/18S Basic combined with Molzym's unique DNA isolation technology, MolYsis™, will lead to a significant increase in the PCR sensitivity.
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Selective Enrichment of Bacterial and Fungal DNA
MolYsis™ is a tool for the removal of human DNA and enrichment of bacteria and fungi from liquid samples. Technically, in a short series of steps, MolYsis™ effects the selective lysis of blood cells and the quantitative degradation (>99%) of released human DNA. After enrichment from the lysate by centrifugation, microorganisms are treated with a reagent degrading cell walls of Grampositive and Gram-negative bacteria and fungi. The broad range lysis potential has been shown in clinical evaluations, including over 200 genera of bacteria (86 Grampositives, 120 Gram-negatives) and 65 genera of fungi.
Hot MolTaq 16S/18S: DNA-Free PCR Reagents
A common drawback of PCR assays for bacterial and fungal sequences is the contamination of amplification reagents by microbial DNA. As a consequence, false-positive results may arise often also associated with a loss of analytical sensitivity. Molzym‘s Mastermix 16S/18S products are guaranteed free of contaminating bacterial and fungal DNA. This provides accurate results also at low target loads.
