Status:
ReviewerMember since: 2020
Organization: Laboratorio de hemoderivados UNC
Superior results!
Application Area: Purification of PCR products
"It is ideal for purifying PCR products from agarose gels or directly from the tube if you need to use them for posterior applications. Yields high purity nucleic acids and with high concentrations."
Status:
ReviewerMember since: 2022
Organization: UC Davis
Our favorite DNA clean up kit!
Application Area:RT PCR product clean up prior to Sanger sequencing
"This is our go-to kit for PCR product clean up for Sanger sequencing. Very convenient to use and cheaper than other DNA clean up kits. We also always get reproducible and reliable results with minimal loss of material."
Status:
ReviewerMember since: 2022
Organization: Baylor College of Medicine
A must have in a lab
Application Area:Molecular biology
"Great kit to purify DNA, especially from PCR. High quality at the end with almost full recovery."
Status:
ReviewerMember since: 2021
Organization: Brown University
Great results.
Application Area:Clean linearized DNA for in vitro RNA transcription
"I achieve high quality pure DNA which means my in-vitro RNA transcription is pure too."
Status:
ReviewerMember since: 2012
Organization: SAINT LEO UNIVERSITY
Cannot praise this highly enough, we won't use anything else.
Application Area:Purify PCR product
"This product is amazingly easy to use and gives reproducible, high quality end product. My students have used this repeatedly and we are yet to be disappointed. From PCR reaction product to purified DNA in 30 minutes or less. Students have then used their purified DNA in cloning reactions and, touch wood, we have yet to see a failure."
Status:
ReviewerMember since: 2022
Organization: UNC Chapel Hill
Great kit and amazing customer service!
Application Area:Purifying DNA to use in DNA-detection based biomarker assay.
"Not only does the clean and concentrator kit increase the quality of my sample, but it also makes me trust my data and enhances reproducibility. The customer service at Zymo is also unmatched. I called for advice on the best kit to use for my specific assay and the Zymo agent I worked with was very thorough in their search for the best product for my needs. They also were awesome at following up via email to make sure that their suggestion was the right fit for my assay."
Status:
ReviewerMember since: 2021
Organization: Root Applied Sciences
My kit for cleaning environmental DNA extracts
Application Area:Cleaning environmental DNA
"Clean & Concentrator kit is very easy to use. Great for trainees with less experience to learn with minimal risk of ruining valuable samples. While other methods may be cheaper, the ease and speed of this kit make it my preferred method for cleaning DNA."
Status:
ReviewerMember since: 2021
Organization: Unsw
Great results being reproduced.
Application Area:Dna cleanup and concentration of multiple samples in one.
"The product is very easy to use. And it has been easy to reproduce results for downstream analysis."
Status:
ReviewerMember since: 2019
Organization: NWP
Great value, highly recommend
Application Area:Plant disease diagnostics
"I've always had great results using Zymo's DNA Clean and & Concentrator kits for PCR purification of samples that will be sent off for sequencing. I really like that you can order the kit components separately."
Status:
ReviewerMember since: 2021
Organization: Scripps Research
Highly recommend!
Application Area:DNA purification for next-generation sequencing
"This kit is easy to use and the protocol is straightforward."
Status:
ReviewerMember since: 2020
Organization: Arcturus
Easy to use, high quality product
Application Area:DNA cleanup for cloning
"This product has been our go to for years. We routinely get clean DNA at a good concentration back for our downstream activities and there has never been any inhibition of downstream steps due to carryover from the cleanup. Highly recommend for ease of use and quality of product."
Status:
ReviewerMember since: 2019
Organization: Yale University
Works better than Qiagen.
Application Area:Large volume gDNA preparation
"Worked great for DNA cleanup after gDNA extraction from large quantities of cells."
Status:
ReviewerMember since: 2021
Organization: University of Hawaii at Manoa
Great product. I would like to recommend it to my colleagues.
Application Area:Sequencing
"The purification kit from Zymo Research is super straightforward to use, and the effectiveness is high. The yield of DNA is also high and pure."
Status:
ReviewerMember since: 2021
Organization: BPS Bioscience
Great results for a low price, and great customer service.
Application Area:Purified concentrated DNA
"I used both the 25µg and the 500µg size of this product. The instructions were easy to follow and I was glad to find the resulting DNA to be both very pure and highly concentrated. We had previously used the Macharey-Nagel products for this purpose, and the Zymo kits worked just as well for us, for a lower price. Their customer support team was excellent to work with. They were very responsive and were able to get a scientist to help with my specialized question very quickly."
Status:
ReviewerMember since: 2000
Organization: Northwestern College
Nearly foolproof!
Application Area:Purify PCR reactions for downstream use
"I use this kit with undergraduate research students. We use this kit to purify PCR products for downstream cloning. The kit is nearly foolproof! My undergrad students (some are even first year college students) consistently get clean DNA, high yield, and use it successfully in downstream cloning reactions."
Status:
ReviewerMember since: 2021
Organization: Brown University
Great results, can't live without it.
Application Area:Clean and concentrate linearized DNA
"It's easy to use and necessary for my experiments to continue."
Status:
ReviewerMember since: 2021
Organization: cinvestav
Excellent results in a short run time.
Application Area:Analysis of bacterial communities in sediment samples
"The product is very effective and high quality results are obtained since it allows to obtain a good purification in PCR products. It is a very easy to use kit and the best thing is that it does not delay in performing the method. After-sales service is very good and delivery times are smooth. The price is good for the product."
Status:
ReviewerMember since: 2020
Organization: University of Washington
Awesome.
Application Area:Plasmid clean up
"So great for concentrating to 6ul, use often for illumina library prep."
Status:
ReviewerMember since: 2020
Organization: University of California
Great! Reliable and best on the market.
Application Area:Cleaning PCR up for cloning reactions
"The DNA cleanup kit from Zymo is wonderful to clean up PCR products for downstream cloning. Never had a problem with the kit and is better than common "premium" cleanup kits I've used. Will be using this product for a long time, it's so reliable!"
Status:
ReviewerMember since: 2020
Organization: University of Connecticut
Useful and reliable for single-cell extraction.
Application Area:Single-cell DNA extraction for PCR
"We work with single-celled marine organisms (ciliates) and it can be difficult (a) to extract ample DNA for downstream PCR application and (b) to eliminate PCR inhibitors present in seawater (ex. seawater itself, to start). We've played around and purchased a lot of different DNA extraction kits and this (and the 5 kit) are the only to reliably extraction ample DNA for PCR amplification."
Status:
ReviewerMember since: 2019
Organization: Iowa State University
Simple to use, saves so much time!
Application Area:PCR purification of DNA fragments
"Compared to other PCR cleanup kits, this saves so much time and results in much concentrated samples. It allows elation in very small volumes."
Status:
ReviewerMember since: 2019
Organization: Instituto Tecnologico de Costa Rica
Cost effective, easy to use, and great results!
Application Area:gDNA library preparation (restriction digest cleaning, sample pooling, library cleaning)
"DNA cleaning is a must during ddRAD library preparation. The kit provides an easy way to eliminate enzymes and by-products after the restriction digest and barcode adapter binding steps or after PCR. I prefer this kit much more than using magnetic beads. This kit is cheaper and just works. You don't have to mess with beads or buffer concentration, nor buy expensive magnetic plates. As a side note, I work with plant tissue, sometimes do to latex, resins, and phenolic compounds – so DNA extraction and purification can be a hassle. Furthermore, depending on the source material (leaf, bark, root, etc...), DNA yield can vary widely. Using this kit has proven to be a cost effective and easy way to clean and concentrate samples, regardless of DNA extraction protocol and source material. DNA recovery from the columns is quite good, with minimal losses after cleaning (based on Nanodrop readings before and after)."
Status:
ReviewerMember since: 2019
Organization: UNC Biospecimen Processing Facility
Lost precious samples due to degradation of the spin columns.
Application Area:Concentration of genomic DNA samples.
"We have not used this kit since 2018 due to the following problems with the Zymo-Spin IIC-XL, which were observed multiple times with multiple lots. Customer service and Zymo techs were very helpful but the processing issue was never resolved. After processing our samples with the Zymo kit, there is a significant decline in the 260/280 and 260/230 values. A white, heterogeneous residue is visible when transferring the eluent from the final collection tube to a 1.5ml screwcap tube. A small, white pellet is visible if samples are spun for 5 minutes at 16,000g. Once the supernatant is removed from the pellet, only slight improvement is observed – and the 260/280 and 260/230 values are still significantly lower than pre-Zymo values. It seems like the spin column filter is degrading and coming through with the eluent. We were unable to continue processing any samples using the Zymo gDNA Clean & Concentrator Kit, as we cannot afford to lose precious sample."
Status:
ReviewerMember since: 2019
Organization: Northwestern
Great!
Application Area:Clean sample during smFISH probe production
"The instructions are simple and easy to follow. The company is willing to help with any issues with the product. I feel that the amount of reagent and materials in the kit is cost effective."
Status:
ReviewerMember since: 2019
Organization: Icahn School of Medicine at Mount Sinai
Great results and critical to our research.
Application Area:Clean up DNA following isolation from blood samples, for future use in DNA methylation analyses
"I regularly achieve high quality results with this product! Any inability to achieve DNA at the end of my concentrating is likely due to errors along the first isolation step attributed to a kit outside of Zymo Research. The kit is very easy to use and great value!"
Status:
ReviewerMember since: 2019
Organization: University of Illinois
I will keep using it.
Application Area:Vector construction
"This is my to-go kit for cleaning DNA for different applications. it is straightforward and consistent. It is also very affordable compared to similar products from other brands."
Status:
ReviewerMember since: 2016
Organization: Georgia Institute of Technology
Very easy to use with good results.
Application Area:Concentrate DNA for PCR
"I have been using the product for a year now. Very easy to use with good results. Highly recommended!"
Status:
ReviewerMember since: 2016
Organization: Pomona College
Good for concentration into small volumes.
Application Area:Clean and concentrate DNA following PCR or Gibson Assembly
"Useful for concentrating DNA product into small volumes (such as for transformation), but showed greater loss of product in direct comparison with other DNA concentration products in my lab."
Status:
ReviewerMember since: 2016
Organization: OHSU
Easy to use, reproducible results and an essential lab kit.
Application Area:Clean and concentrate DNA samples
"Zymo Clean and Concentrator kit yields reproducible results and is extremely quick. I don't notice a yield loss when adding a sample to this column in order to exchange buffers or concentrate my DNA samples."
Status:
ReviewerMember since: 2016
Organization: University of California, San Diego
Great kit, easy to use, and everyone in the lab uses it.
Application Area:DNA cloning, PCR cleanup, primer removal.
"The kit is easy to use and gives consistent results. Useful for cloning and other molecular biology applications. Good to concentrate DNA with elution in small volumes."
Status:
ReviewerMember since: 2016
Organization: UT Austin
It does the job it says it can.
Application Area:I use this kit to concentrate and clean up PCR for genome engineering purposes.
"Excellent value kit to concentrate your sample. It is effective and usually gives high yields."
Status:
ReviewerMember since: 2016
Organization: Michigan State Univeristy
For the price and ease, it’s great to work with.
Application Area:Clean and concentrate DNA after extraction from human tissues
"The product is very easy to use. The best part is able to elute in small volumes which is very beneficial. The only caveat is the loss after clean up. I have experienced 40-50% loss and would like more recovery. DNA has been used for downstream applications such as bisulfite analysis and exome sequencing and the data was very clean."
Status:
ReviewerMember since: 2016
Organization: Duke University
Works really well -- I suggest this over other clean-up kits.
Application Area:DNA purification after restriction digest
"The clean and concentrator kit is simple to use, with quick-to-use instructions included in the box. Cleaning up the DNA takes <7 minutes from addition to the column to elution. The best part is being able to control the volume of elution from the column, with 6 µL the minimum! Now I can get concentrated DNA to use for in vitro transcription."
Status:
ReviewerMember since: 2016
Organization: Singlera
Very easy to use with high recovery
Application Area:Purification of dna
"Very easy to purify DNA for ngs. High quality results with great recovery. I would recommend this kit over other columns"
Status:
ReviewerMember since: 2016
Organization: University of Connecticut
Love Zymo kits, they are my favorite.
Application Area:Clean DNA for PCR and sequencing
"Zymo products are great. The kits have easy workflows and are well organized when shipped. The protocols are also nice and simple to follow and if I ever have any questions, the support staff is very helpful."
Status:
ReviewerMember since: 2016
Organization: Lineberger Comprehensive Cancer Center
Nice results overall
Application Area:DNA purification from enzymatic reaction
"Its gives high yield and its pretty easy to use and need very less time."
Status:
ReviewerMember since: 2016
Organization: Yale
Love this product!
Application Area:cloning of bacterial expression constructs
"This is a wonderful product! Easy to use and produces great results each time!"
The DNA Clean & Concentrator™-25 (DCC™-25) is designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates.
Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin™ column.
The product features Fast-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.