DNA Clean & Concentrator™-25 by Zymo Research

"We work with single-celled marine organisms (ciliates) and it can be difficult (a) to extract ample DNA for downstream PCR application and (b) to eliminate PCR inhibitors present in seawater (ex. seawater itself, to start). We've played around and purchased a lot of different DNA extraction kits and this (and the 5 kit) are the only to reliably extraction ample DNA for PCR amplification."

"Compared to other PCR cleanup kits, this saves so much time and results in much concentrated samples. It allows elation in very small volumes."

"DNA cleaning is a must during ddRAD library preparation. The kit provides an easy way to eliminate enzymes and by-products after the restriction digest and barcode adapter binding steps or after PCR. I prefer this kit much more than using magnetic beads. This kit is cheaper and just works. You don't have to mess with beads or buffer concentration, nor buy expensive magnetic plates. As a side note, I work with plant tissue, sometimes do to latex, resins, and phenolic compounds – so DNA extraction and purification can be a hassle. Furthermore, depending on the source material (leaf, bark, root, etc...), DNA yield can vary widely. Using this kit has proven to be a cost effective and easy way to clean and concentrate samples, regardless of DNA extraction protocol and source material. DNA recovery from the columns is quite good, with minimal losses after cleaning (based on Nanodrop readings before and after)."

"We have not used this kit since 2018 due to the following problems with the Zymo-Spin IIC-XL, which were observed multiple times with multiple lots. Customer service and Zymo techs were very helpful but the processing issue was never resolved. After processing our samples with the Zymo kit, there is a significant decline in the 260/280 and 260/230 values. A white, heterogeneous residue is visible when transferring the eluent from the final collection tube to a 1.5ml screwcap tube. A small, white pellet is visible if samples are spun for 5 minutes at 16,000g. Once the supernatant is removed from the pellet, only slight improvement is observed – and the 260/280 and 260/230 values are still significantly lower than pre-Zymo values. It seems like the spin column filter is degrading and coming through with the eluent. We were unable to continue processing any samples using the Zymo gDNA Clean & Concentrator Kit, as we cannot afford to lose precious sample."

"The instructions are simple and easy to follow. The company is willing to help with any issues with the product. I feel that the amount of reagent and materials in the kit is cost effective."

"I regularly achieve high quality results with this product! Any inability to achieve DNA at the end of my concentrating is likely due to errors along the first isolation step attributed to a kit outside of Zymo Research. The kit is very easy to use and great value!"

"This is my to-go kit for cleaning DNA for different applications. it is straightforward and consistent. It is also very affordable compared to similar products from other brands."

"I have been using the product for a year now. Very easy to use with good results. Highly recommended!"

"Useful for concentrating DNA product into small volumes (such as for transformation), but showed greater loss of product in direct comparison with other DNA concentration products in my lab."

"Zymo Clean and Concentrator kit yields reproducible results and is extremely quick. I don't notice a yield loss when adding a sample to this column in order to exchange buffers or concentrate my DNA samples."

"The kit is easy to use and gives consistent results. Useful for cloning and other molecular biology applications. Good to concentrate DNA with elution in small volumes."

"Excellent value kit to concentrate your sample. It is effective and usually gives high yields."

"The product is very easy to use. The best part is able to elute in small volumes which is very beneficial. The only caveat is the loss after clean up. I have experienced 40-50% loss and would like more recovery. DNA has been used for downstream applications such as bisulfite analysis and exome sequencing and the data was very clean."

"The clean and concentrator kit is simple to use, with quick-to-use instructions included in the box. Cleaning up the DNA takes <7 minutes from addition to the column to elution. The best part is being able to control the volume of elution from the column, with 6 µL the minimum! Now I can get concentrated DNA to use for in vitro transcription."

"Very easy to purify DNA for ngs. High quality results with great recovery. I would recommend this kit over other columns"

"Zymo products are great. The kits have easy workflows and are well organized when shipped. The protocols are also nice and simple to follow and if I ever have any questions, the support staff is very helpful."

"Its gives high yield and its pretty easy to use and need very less time."

"This is a wonderful product! Easy to use and produces great results each time!"