Direct-zol™ RNA Microprep by Zymo Research

"DirectZol by Zymo is a great product that has drastically increased the effectiveness of RNA purification of samples. It has allowed us to obtain more RNA in a shorter amount of time, and has overall streamlined the process of RNA extraction for downstream qPCR application."

"I contacted their service about our organisms. They provided good ideas and the yield is good. We have been using this for couple years now. Nice and simply cut down work time for RNA extraction a lot compared to traditional Trizol protocols."

"We got very high quality yield RNA compared to traditional Qiagen extraction kits. Much easier than phenol/chloroform extractions from TRIzol. Highly recommend. using this product!"

"This product was very easy to use. For RNA extraction from a marine sediment, I tested several protocols and kits and with Direct-zol RNA Microprep I obtained the best quality of RNA."

"It is fast, easy to use and we get quality results."

"I manually extracted RNA from the cells and tissues, and sometimes had issues with RNA quality, especially when I extracted RNA from the cells (e.g., low A260/A230 ratio). So we decided to use the Direct-Zol RNA Microprep. The kit is easy to follow and I got enough amount of RNA for qPCR. I tried both 12-well and 24-well plate-seeded cells, and kit successes to give me enough amount of RNA with good quality."

"This kit is very user friendly and simple to use. The protocol is fast, but this does not compromise the quality of the RNA that is extracted. I highly recommend this kit, particularly for high throughput extraction."

"Zymo Direct-Zol RNA Microprep is a game changer in our workflow. The ease of use, amazing yield and purity of RNA is unmatched. It has significantly increased our workflow and productivity. The samples are reproducible and reliable."

"This is an excellent product for extracting high quality RNA quickly and easily. I have used RNA extracted with this kit for RNA-Seq and I got great data."

"Achieve high-quality results using this! I have tried different kits and this one gives me the best results. It is easy to use and the whole process is pretty quick!"

"I have been using Direct-Zol for more than a year now and it is a great alternative to otherwise difficult phenol-chloroform extractions. Instead of labor-intensive and skilled phase separation, the entire sample can be isolated on a column and carried out as a regular extraction. This kit gives high yield, high quality, and reproducible results in a much more efficient way than traditional Trizol extractions, but still allows the user to preserve samples in Trizol."

"I was the first one to try a single individual (~1 mm) RNA-seq in the lab and the RNA quantity and quality are critical to the success of my experiment. I searched online for a while to look for an RNA extraction kit and I felt Directzol microprep looks like a good fit, so I gave it a try. It is convenient and fast, and most importantly, it gave me success for my first single individual RNA-seq! Still use it now!"

"This kit saves so much time in comparison to their competitors - I'm able to process up to 24 samples in 1-1.5 hours. With the experiments I commonly undertake (usually up to 100 samples/ea), that is a HUGE time saver. And the price can't be beat, especially given how simple the isolation procedure is and the great reproducibility of the results. We are a multi-faceted lab that uses this kit to not only isolate RNA from human cell lines, but also from tick and fruit fly cell lines and whole or dissected tick tissues. Very versatile! I've never had an issue with the company either, which has always been an exceptional interaction!"

"RNA quality is sufficiently high for downstream applications (functional assays as well as molecular biology). We changed protocol a little and use only the H2O-phase after phenol-chloroform separation for isolation. Also we added a required addition of the DNAse digestion step to the protocol. Our RNA is almost completely DNA free."

"I found it very easy to use. User friendly, outstanding results. Got a grant using results from this micro RNA kit. Got a promotion."

"This product is extremely effective for obtaining high quality RNA from tissue. We used brain and spinal cord for obtaining RNA for qPCR experiments and the results were excellently. The kit was easy to use and instructions were clear and provided alternatives should we need to change the protocol to fit our needs. Zymo Research is quick to respond to needs and orders. The price of the kit was well worth it as the RNA was of high quality and we could extract and purify it with ease thanks to the kit."