DeepSIM is the fist high-end, accessible SR-SIM (super resolution structured illumination microscope) module, compatible with up-right or inverted microscope, as easy as a confocal. To guarantee a seamless evolution from Confocal to SR, DeepSIM doesn’t require any special probes and researchers can maintain unaltered their standard sample preparation protocols. The system is equipped with robust system calibration, pre-optimized acquisition modalities and automated data processing to break down the learning curve.
Aware that establishing SR-SIM as a common tool for routine life science research applications requires much more than ergonomic design and intuitive handling, DeepSIM has been developed to be a truly enabling technology, where performance is combined with flexibility and advanced applications integration.
The DeepSIM enables to pair spatial super resolution (100nm in lateral X,Y and 300nm axial Z), equivalent to an ~8-fold volumetric improvement, with high temporal resolution. To enable ultra-rapid dynamics studies, the DeepSIM can be run in Fast Speed mode to reach >10fps acquisition framerate with the Large FOV (1024x1024px), thus making the DeepSIM particularly geared towards 4D live-cell imaging and higher throughput applications.
A major handicap of all SR methods is their susceptibility to aberrations, especially when imaging deeper than 10 µm, which impacts contrast and resolution. To ensure the maximum confidence in the data collected even in depth, the DeepSIM reconstruction algorithms are designed to work with samples of thickness comparable to what can be used with confocal microscopy, taking in consideration the scattering of light. Thanks to this innovation, DeepSIM allows deeper SR imaging into (live) biological tissues and organisms over 50µm thick. The enhanced optical sectioning capacity can be obtained, not only using high magnification objective (60X, 100X), with also low magnification objectives (20x-40x) to expand as much as possible the volumetric context of your acquisitions.