Member since: 2019
Organization: Cardiff University
Simple and easy to generate quality results.
Application Area: Assess LDH release in vitro
"Excellent product provided with comprehensive instructions that are easy to follow. Easy to scale up or down, sensitivity was satisfactory."
Member since: 2016
Organization: Charles University in Prague
Good product and great results!!!
Application Area:Quantification of LDH in medium of in vitro cultures
"The assay is very precise and sensible. It allows good quantification of cytotoxicity in organotypic cultures by quantification of LDH in the medium."
The assay rapidly measures the release of lactate dehydrogenase (LDH) from cells with a damaged membrane. LDH released into the culture medium is measured with a 10-minute coupled enzymatic assay that results in the conversion of resazurin into a fluorescent resorufin product. The amount of fluorescence produced is proportional to the number of lysed cells using a 96- or 384-well format. The CytoTox-ONE™ Reagent does not damage normal healthy cells; therefore the reactions set up to measure released LDH can be performed directly in a homogeneous format in assay wells containing a mixed population of viable and damaged cells.
The CytoTox-ONE™ Homogeneous Membrane Integrity Assay, HTP (Cat.# G7892), offers convenient, alternative packaging for processing multiple plates. Each bottle of reagent supplied with the system is sufficient to perform 500 assays in a 96-well format or 2,000 assays in a 384-well format when the recommended volumes are used.
Save time by setting up the assay in a cell culture plate; this eliminates the sample transfer step common in many LDH assays. Plates are then incubated for just 10 minutes before reading data, compared to 30 minutes or more with classic LDH assays.
Multiplexing capability allows you to perform multiple assays on one sample with other homogeneous cell-based assays from Promega.
Adapt the assay protocol to your needs; completed assays can be read over several hours after the provided stop solution has been added while still maintaining good signal.