CytoFLEX Flow Cytometer by Beckman Coulter Life Sciences

"Very easy to use. Can be used for a wide range of applications."

"Cytoflex S allows an easy way to analyze extracellular vesicles (EV) with its impressive 405-SSC and fluorescence sensitivity. Using beads for calibration, it's able to detect 80um beads without problems, but this is not the real thing, as we know. The real thing is to work with biological particles of known size (defined by other technique, DLS, Nanosight or EM) and use them as size standards. We use phages as standard (from 50nm to 150 nm) and Cytoflex S is able to discriminate them from noise easily, therefore, allowing us to analyze EV without problems. It's fluorescence sensitivity lets us detect low epitope EVs. Also is possible to differentiate by scattering of different EVs populations. Its use is very simple, can use 96 well plates, the software is very straightforward and the price, keeping in mind that is a 4 laser/13 fluorescences instrument, is very competitive."

"The CytoFlex has won me over! I was a little sceptical of voltage free detectors, but the users have found this so easy to use, that I realise that I may have been a bit conservative in my own mind. The biggest bonus has been the increased sensitivity which has increased the ease of analysis through being able to discriminate weak positive samples. Another key change for us is the ability to use it for single tubes or for plates. In a multiuser core facility, this has increased the user base significantly and results in long sessions using the plate loader freeing up the user to carry on with other research. Indeed, they often add the plate at the end of the day, go home ad then collect the data in the morning. We bought the 'S' and then followed up with 5 'LX' systems which are now distributed across East Africa for a collaborative study, with one remaining at York ad already become the most user system over all others."

"This machine is a game changer, the stable, fast and accurate performance of this machine really accelerated my work."

"The Cytoflex we use present 4 channels through the use of one laser. This allows us to detect the fluorophores, FITC, PE, Cy7, Cy5.5. Compensations on this machine are very intuitive and can be achieved fairly easily. reproducibility is not an issue as all our error bars are minimal."

"The Cytoflex is a benchtop flow cytometer with a small foot print. This is a highly sensitive instrument as the detectors are based on APD (avalanche photo diode) technology. It is easy to set up and students can start using it within an hour of training. The data acquired is compatible to most third party data analysis software suppliers. I would highly recommend it to a teaching or research lab. "

"Easy to use instrument with lots of flexibility for different experiments. Excellent instrument, highly recommend."

"Product and service was very great. Easy to use and very user friendly. Data produced is reproducible and of high quality. No false positives. Easy to train others. I give it 5 star ratings. It is worth the money as well."

"We have owned one for just over a year, cost ~$50k for a two laser, 5 color system with plate loader. Very easy to use, quick to set up in just a few minutes and at least as sensitive as our core's FACSAria III. Filters are easily replaceable if you have a specific application but works great out out of the box. I can have members of staff trained on it in just a few minutes. Minimal downtime so far, mainly from poor cleaning as the manual does not have a clear daily startup and shutdown protocol. I would recommend buying a multiple probes and peristaltic tubing as these are the only consumables. We love it, use it daily, and I would buy it again. The software is a hybrid of Diva and FlowJo and produces reasonable quality images so no subsequent analysis required."

"We have used the cytoflex for over a year both for human and mouse cells. Along with standard surface staining of blood and bone marrow cells, we have undertaken studies of phospho-flow signalling, cell cycle, apoptosis, ROS measurements, apoptosis and viability and genetic reporter expression (GFP, YFP, dsRed) "

"This is a fantastic flow cytometer. We have chosen it among 5 tested and it is definitively at the cutting edge of technology. The software is totally impressive and acquisition is really accurate, reproducible and fast."

"Cytoflex is easy to use, give good results immediately, is versatile, and cheap, so the value for money is high. It is a technological innovation in the world of flow cytometry. Its sensitivity allows to work in nano detection dimension, never allow to flow cytometry."

"Cytoflex is the new flow cytometer of Beckman Coulter portfolio. Is very easy to use it because software is really user friendly. The instrument can be used after 15 minutes of the parcel arriving. Its sensitivity is amazing and it is very versatile, it can be used for microvesicles analysis, microbiology applications, immunophenotyping, plant ploidy and many more applications. Normal configuration is 3 lasers (405, 488, 640 nm) and 13 fluorescence detectors. Also can be equipped with a plate loader (but it needs some improvements). Its price is very affordable for such a high value instrument. It has almost no maintenance. "