Cyto-Mine®
Cyto-Mine® - The Single Cell Analysis and Monoclonality Assurance System. Cyto-Mine® is a fully automated platform which integrates high-throughput and selective single cell screening, sorting, isolation and monoclonality assurance using high quality imaging.
Cyto-Mine® System
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The Cyto-Mine has revolutionised our CLD and made a big difference to our timelines.
Selection of high productivity cells expressing antibodies
We use the Cyto-Mine in our platform process in cell line development. It allows us to screen for the higher productivity cells and remove the low productivity cells in a cell pool, and then dispense these into 96/384 well plates. The assays work well across a range of antibody and novel formats. The Cyto-Mine has definitely reduced our timelines, and we have full confidence it will find the highest expressing cells from a pool. It also gives great insight by showing the diversity of expression cells in a pool or how a clone cell population changes over time.
Review Date: 26 Jul 2023 | Sphere Bio
Excellent instrument, using innovative technology.
Cell line development
We have integrated the Cyto-Mine into our cell line development workflow which has allowed us to shorten our timeline without compromising on the quality of the cell lines that we generate. We have found the instrument to be very user friendly. The support offered by Sphere Fluidics has been excellent and we are very happy with the purchase. The ability to image the picodroplets prior to dispensing into a 96-well plate together with a 'heat-map' of the 96-well plate is a feature that we find very useful.
Review Date: 6 Sept 2019 | Sphere Bio
Sphere Bio's Cyto-Mine® is a fully integrated platform for high-throughput single cell analysis based on microfluidic picodroplet technology. By encapsulating single cells in picolitre volume droplets, Cyto-Mine® enables the rapid analysis, selective screening, sorting, imaging and dispensing of single cells into individual wells of microtiter plates. A key enabling component of Cyto-Mine® is its ability to analyze and measure secreted proteins or other biomolecules while maintaining high cell viability.
The unique workflow of Cyto-Mine® offers a solution to resource-intensive, time-consuming and expensive workflows that require multiple items of equipment by streamlining cell isolation, titer determination and assurance of a single cell progenitor while reducing timelines and costs for biologics discovery and development.
Key Features:
- Encapsulate up to 200,000 single cells or up to 40 million cells in mini-pools in one experimental run
- Assay secreted proteins or biomolecules e.g. assess cell line productivity or find rare cells in large heterogenous populations
- Selectively identify and sort ‘hit’ cells
- Image each individual cell for assurance of monoclonality to meet regulatory requirements
- Dispense individual cells directly in to 96- or 384-well microtiter plates
