Member since: 2019
Organization: Baylor College of Medicine
GREAT basement membrane - our tissue culture cells are happy, and so are we
Application Area: Bacement membrane for tissue culture
"We use/used Corning Matrigel for all our tissue culture needs, but since the pandemic, their supply chain has been WACK - i.e. we never know when we are getting more, and we cannot get the amount we need to keep our tissue culture work going. So, we have been hunting long and are to find a basement membrane alternative that our cells will like. After trying many samples, we found this one works great! The prep time is a bit longer than when working with Matrigel, and you have to store it at -80 (vs-20), but it works just fine and we can actually get a steady supply!"
Why use Cultrex RGF BME, Type 2?
Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.
Cultrex RGF BME, Type 2 provides a proprietary formulation that is high in tensile strength and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.
Protocols utilizing Cultrex RGF BME, Type 2 for organoid cell culture
Cultrex RGF BME, Type 2 is ideal for use as a scaffold for organoid and 3D cell culture. Listed below are protocols designed by our research and development groups for organoid culture featuring Cultrex RGF BME, Type 2 as well as growth factors, media supplements, and small molecules from Bio-Techne.