Criterion™ TGX Stain-Free™ Precast Gels by Bio-Rad
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The new Criterion™ TGX (Tris-Glycine eXtended) Stain-Free™ precast gels for PAGE are based on the long shelf life TGX formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with the Gel Doc™ EZ and ChemiDoc™ MP imaging systems.
Benefits include:
- Fast run times with protein separation, gel imaging, and complete analysis in less than 30 min
- Rapid fluorescent detection with the Gel Doc EZ and ChemiDoc MP imaging systems
- Comparable sensitivity to Coomassie stain
- Better reproducibility and quantitation compared to other staining procedures
- Inexpensive Laemmli buffer system, low running costs
- Protein transfer in as little as 15 min
- Same gel can be used for staining, western blotting, or mass spectrometry
TGX Stain-Free gels retain Laemmli-like separation characteristics using standard sample and Tris/Glycine running buffers. The proteins in the gel can be separated in as little as 20 min using the Criterion electrophoresis cell and then visualized with the Gel Doc EZ and ChemiDoc MP imagers in as little as 2.5 min. The trihalo compounds react with tryptophan residues in a UV-induced reaction to produce fluorescence, which can be easily detected by the Gel Doc EZ and ChemiDoc MP imagers within gels or on a PVDF membrane after transfer.
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