CRISPRi dCas9 mRNA
Horizon's proprietary dCas9-SALL1-SDS3 CRISPRi repressor in mRNA format
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Co-transfect dCas9 mRNA with synthetic CRISPRi guide RNA for a lentiviral-free gene repression workflow.
CRISPR-mediated transcriptional repression poster
First-generation CRISPRi systems utilize the Krüppel associated box (KRAB) domain from zinc finger protein 10 (KOX1) fused to dCas9 (dCas9- KRAB) as a transcriptional repressor, an approach shown to be more target-specific than other existing technologies for gene repression. Given that this CRISPR-based approach can also result in less robust repression of the target gene(s), there is a need for identifying ways to improve its efficiency. Download this poster by Horizon Discovery to find out the results of its study on CRISPR-mediated transcriptional repression.
CRISPR without the cut: a novel CRISPR interference system enabling rapid functional gene characterization
This webinar will highlight the Dharmacon™ CRISPRmod CRISPRi system as detailed in a peer-reviewed article in The CRISPR Journal.
Learn first-hand from the inventor about its proprietary effector specifically developed for use with synthetic single guide RNAs (sgRNAs). The system enables functional gene characterization in arrayed format experiments making it a powerful, orthogonal method to siRNA and CRISPR knockout screening.
Key learning objectives
- Learn about a novel CRISPRi effector, dCas9-SALL1-SDS3, that mediates more potent repression when used with synthetic sgRNAs than previously described effectors such as dCas9-KRAB and dCas9-KRAB-MeCP2.
- Discover how dCas9-SALL1-SDS3 interacts with key members of the histone deacetylase and Swi-independent 3 complexes to mediate rapid, potent target gene repression.
- Learn about the development of in vitro-transcribed dCas9-SALL1-SDS3 mRNA for delivery into clinically relevant cell types such as human induced pluripotent stem cells and primary T cells.
- Explore the functional gene characterization of DNA damage host factors using dCas9-SALL1-SDS3 with synthetic sgRNAs, demonstrating the ability of the system to be used in arrayed-format screening.
Who should attend?
- Academics and industry professionals interested in functional genomics, specifically gene editing and gene modulation (CRISPR-Cas9 and RNAi technologies)
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
