CRISPR-Ready ioGlutamatergic Neurons™
Human iPSC-derived glutamatergic neurons expressing Cas9 for rapid gene knockout generation
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Ease of use, great support, CRISPR ready
Neuronal culture, CRISPR
I had done a few genome wide CRISPR screens in immortalized mammalian cell lines and wanted to do similar experiments in human primary neurons. The complexity of iPSC derived neuronal cultures was putting me off this intriguing experiment. With Bitbio's cells, it turned out to be a breeze. The support team was really helpful and gave me all the detailed advice. The product was delivered soon and doing the pilot experiments has been nothing like the sort of mess I had been warned about. Hopefully, it will get me exciting results.
Review Date: 16 Jan 2024 | bit.bio
Reproducible results and suitable for high throughput screening
Neuroscience
A very much need addition to the field for functional studies
Review Date: 16 Jan 2024 | bit.bio
Great results, can’t live without this instrument
Regenerative medicine
I am a researcher who is investigating the function of glutamate receptors in synaptic plasticity using CRISPR-Ready ioGlutamatergic Neurons (CRISPR-ioGluNs). CRISPR-ioGluNs have shown to be really successful in my application. They are simple to use and have considerably increased my capacity to regulate glutamate receptor activation. I am also quite pleased with the after-sales service provided by the maker of CRISPR-ioGluNs. They were quick to respond to my inquiries and concerns, and they offered good technical assistance. Overall, I strongly advise other researchers seeking a diverse and useful method for investigating the nervous system to use CRISPR-ioGluNs.
Review Date: 16 Jan 2024 | bit.bio
CRISPR-Ready ioGlutamatergic Neurons are built from our well-established wild type ioGlutamatergic Neurons™, engineered to constitutively express Cas9 nuclease. These cells arrive ready for guide RNA (gRNA) delivery by day 1 post-thaw. Using our optimised lentivirus or lipid-based gRNA delivery protocol, users can maximise their knockout efficiency and start measuring readouts from gene knockouts and CRISPR screens within days.
CRISPR-Ready ioGlutamatergic Neurons arrive ready to use for functional genomics, disease model generation, drug target identification and fundamental human biology research. The cells have been precision reprogrammed from human induced pluripotent stem cells (iPSC) using opti-ox™ technology, meaning scalability and consistency are built-in. In days, they convert consistently to mature, functional glutamatergic neurons characterised by >80% expression of glutamate transporter genes VGLUT1 and VGLUT2.
Users can significantly cut experimental timelines by no longer needing to spend months engineering and characterising their own Cas9 stable iPSC lines or optimising differentiation protocols. With these cells, robust experimental readouts can be achieved by simply delivering gRNAs against your target gene. Users do not require prior expertise in iPSC differentiation or gRNA delivery optimisation.
