Purity > 95% by SDS-PAGE
Shown to promote attachment and spreading of HT-1080 human fibrosarcoma cells
Tested for formation of a firm gel by exposure to ammonia vapors from solutions of 0.5 mg/mL. Further dilution will decrease the rigidity of the gel. NOTE: This preparation contains native collagen molecules with a small amount of nicked or shortened sequences due to pepsin treatment.
Tested and found negative for bacteria, fungi, and mycoplasma
Liquid in 0.012 N HCl
Preparation and Storage
Stable for at least three months at 2° to 8°C. Do not freeze.
Effective as a gel or thin coating at a recommended concentration of 5-10 µg/cm2 of growth surface depending on cell type