Click-IT™ AHA (L-Azidohomoalanine) by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific  |   Model: C10102
5.0
/
5.0
  |  1 reviews
Click-iT® AHA (L-azidohomoalaine) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional radioactive technique, 35S-methionine for the detection of nascent protein. AHA is an amino acid analog that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click " reaction betwe... Read more


Click-IT™ AHA (L-Azidohomoalanine) by Thermo Fisher Scientific product image
Click-IT™ AHA (L-Azidohomoalanine)

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Average Rating: 5.0
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Expert Reviewer
  • Member since: 2013

  • Organization: Cincinnati Children's Hospital Medical Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use and work great!
Rating: 5.0

  • Application Area: Measurement of Nascent protein synthesis

"Click-iT AHA was used to measure nascent protein synthesis in hepatocyte and MEF cells. It is easy to use and works well. The results are reliable and consistent."

Review date: 20 Nov 2017 | Click-IT™ AHA (L-Azidohomoalanine)

Click-iT® AHA (L-azidohomoalaine) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional radioactive technique, 35S-methionine for the detection of nascent protein. AHA is an amino acid analog that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click " reaction between an azide and an alkyne where the azido modified protein is detected with one of the Click-iT® Protein Analysis Detection Kits containing either TAMRA, Dapoxyl®, or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis or Multiplexed Proteomics® reagents for differential analyses of newly synthesized proteins together with total glycoproteins, total phosphoproteins or total protein.