Products & ReviewSeparations

Clean-Up® Hydrophobic Columns

These sorbents are composed of a silica backbone bonded with hydrocarbon chains. They are used to extract compounds, which exhibit non-polar or neutral characteristics, from complex matrices. The C18 phase is the most popular phase.  UCT offers a range of hydrocarbon chain lengths from C2 to C30. In addition, we offer isobutyl, tertbutyl, phenyl, and cyclohexyl bonded phases.

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Description

These sorbents are composed of a silica backbone bonded with hydrocarbon chains. They are used to extract compounds, which exhibit non-polar or neutral characteristics, from complex matrices. The C18 phase is the most popular phase.

UCT offers a range of hydrocarbon chain lengths from C2 to C30. In addition, we offer isobutyl, tertbutyl, phenyl, and cyclohexyl bonded phases.

Application NoteSeparations

Determination of Anabolic Steroids in Horse Urine by SPE and LC-MS/MS

Anabolic steroids are used illegally in horse racing to enhance performance. The compounds are excreted in urine either as glucuronide or sulfated conjugate. To test for steroids in horse urine samples undergo an enzymatic deconjugation to release the parent compounds before extraction. Working with horse urine matrix is challenging and generally unpleasant.


Application NoteSeparations

Comparison of SPE vs. SLE for the Quantitative Analysis of Anabolic Steroids in Serum Using LC-MS/MS

Analysis of anabolic steroids in serum typically requires a sample pretreatment step such as solid-phase extraction (SPE) prior to instrumental analysis. Traditional SPE-based methods utilize C18 or alternative reversed phase sorbents to retain the hydrophobic steroids. Alternatively, the use of a supported liquid extraction, or SLE, is also gaining popularity for this panel of compounds. This application note demonstrates a method that utilizes a traditional reversed phase interaction, but also features the addition of strong-anion exchange functionality (QAX) within the sorbent. This added functionality aids in the removal of unwanted matrix components commonly found in serum such as amino acids and inorganic ions.

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