Agilent BioTek AutoScratch Accessory & Supplies
For use with: Cytation 5, Lionheart FX, Cytation 1, Lionheart LX
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The Agilent BioTek AutoScratch wound-making tool automatically creates reproducible scratch wounds in cell monolayers grown in 24- or 96-well microplates for cell migration and invasion studies. AutoScratch automates the sample prep for imaging using BioTek Cytation cell imaging multimode readers and Lionheart automated microscopes.
The Scratch Assay App automates image collection and analysis of wound width, wound confluence, and maximum wound healing rate.
Advanced wound healing assay workflow using automated scratch wound creation
In this application note, BioTek demonstrates the use of a novel, automated tool to create consistent and reproducible scratch wounds in cell monolayers formed on the bottom of a microplate well. This application note highlights the use of a single cancer cell model as well as a co-culture of fibroblasts and cancer cells; each plated in collagen-coated microplate wells to more closely simulate in vivo microenvironments and facilitate cell migration.
Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays
The wound healing or "scratch" assay is one of the most highly used in vitro methods to monitor and quantify collective cell migration. The current standard involves manual wound creation, which yields low reproducibility between wounds, high variability within generated data, and possible false conclusions regarding test molecules. Using an automated wound creation tool, in addition to kinetic image capture and analysis, repeatable wounds and robust and repeatable results are easily attained.
