Member since: 2016
Organization: University of North Carolina at Charlotte
Great results and highly sensitive system for gene expression analysis.
Application Area: Generate the various droplets for gene expression analysis
"Excellent system if you are working in a field where gene quantification needs to be measured with high sensitivity in an accurate manner. Great machine, easy to handle and to maintain. I am grateful to have this system in our workplace for the great results."
Member since: 2018
Organization: UNC Charlotte
Pretty good instrument for multiple applications, easy to use.
Application Area:Testing a variety of samples for ddPCR analysis
"The droplet generator helps precise analysis with clear droplet units. When it is working well, it can take a lower limit of DNA concentration for successful testing. It is easy to use since all of the steps of droplet generation are automated after you start your run. However, it occasionally reports error messages and takes multiple running cycles to recover from causing an error."
The AutoDG Instrument partitions each sample in a 96-well PCR plate into ~20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Every droplet serves as an individual PCR reaction in Bio-Rad’s Droplet Digital (ddPCR™) PCR System. After partitioning, the plate is sealed with the PX1™ Plate Sealer, and PCR is run to end point in a thermal cycler. The droplets in each sample can then be analyzed using a QX100 or QX200 Droplet Reader.
The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time: it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.
For extended product descriptions and detail specification information, please visit Bio-Rad.com.