Member since: 2018
Organization: The Ohio State University
Great products. But a little bit expensive.
Application Area: Protein digestion
"It gave high-quality results with good reproducibility."
Member since: 2017
Organization: Mitchell Cancer Institute
Application Area:Protein digestion for MS analysis
"Good specificity and works in a broad pH range. Typically a quick digestion works fine."
This endoproteinase hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues (Asp and Cys). Asp-N activity is optimal in the pH range of 4.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in solution or in gel.