Use midi Criterion TGX Stain-Free Protein Gels with IPG/prep wells for the second dimension after isoelectric focusing (IEF) when using 11 cm IPG strips, or for preparative electrophoresis. The novel formulation of TGX (Tris-Glycine eXtended) Stain-Free Protein Gels delivers a long shelf life and allows rapid fluorescent detection of proteins with Bio-Rad’s stain-free enabled imaging systems. TGX Stain-Free Gels can be used for either standard SDS denaturing protein separations or native electrophoresis.
Bio-Rad offers a wide range of Criterion TGX Stain-Free Precast Protein Gels, including other percentage gels and gels with IPG/prep wells, as well as gels with a 700 μl prep well and 2 reference wells.
Run up to four gels using the Criterion Cells; or for higher throughput the Criterion Dodeca Cell can run up to 12 gels or IPG strips.
Criterion TGX Stain-Free Gels contain trihalo compounds, which in the presence of UV-light react with tryptophan residues producing fluorescence. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting, and may be visualized in gels or multiple times on PVDF membranes.
Criterion Precast Gels Application Guide, Instruction Manual