Recombinant S. pyogenes Cas9 nickase, purified from an E. coli strain expressing the nickase. Contains nuclear localization sequence (NLS) and C-terminal 6-His tag. Alt-R S.p. Cas9 D10A Nickase V3 creates a single-stranded cut in the targeted strand of DNA, and Alt-R S.p. Cas9 H840A Nickase V3 creates a single-stranded cut in the non-targeted strand of DNA. Provided in solution at 10 µg/µL. 100 µg of Cas9 nickase = 610 pmol.