Advanced TC Cell Culture Vessels
Vessels with a novel polymer modification enhancing the adhesion and improving the cultivation of fastidious cells
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Perfect design.
Cell culture
Perfectly designed to carry out cell culture experiments with very low chances of contamination.
Review Date: 23 Jul 2021 | Greiner Bio-One GmbH
For the propagation of fastidious cells like primary or sensitive cells as well as cells cultivated under restricted growth conditions (serum-free or serum-reduced) Greiner Bio-One offers the Advanced TC polymer modification.
Based on an innovative technique, the cell culture surface is modified to promote cellular features and functions. Enhanced cell attachment and higher proliferation rates improve and accelerate cell expansion. Furthermore, the Advanced TC surface facilitates consistent and homogenous cell attachment increasing the overall cell yield and reducing cell loss, for example during automated washing steps. Moreover, cells cultivated on the Advanced TC surface exhibit higher transgene activity after gene transfer/insertion. Handling is simplified because transport and storage can be carried out at room temperature. In comparison to biologically treated surfaces, Advanced TC products have a far longer shelf life of 2 years.
Applications:
- Cultivation of fastidious and sensitive cells
- Usage of serum-reduced or serum-free media
- Differentiation of semi-adherent cells
- Transfection
- Transduction
- Automation/High-throughput analysis
Selection of Cell Culture Surfaces for the Adipogenic Differentiation of Human Mesenchymal Stem Cells (hMSC)
This application note describes an experiment to analyse the influence of various commercially available cell culture surfaces on the differentiation of human mesenchymal stem cells into adipocytes within 384 well clear bottom microplates frequently used for high-content and high-throughput screening. The Greiner Bio-One Advanced TC™ surface displayed the highest cell growth and differentiation rate.
Selection of Cell Culture Surfaces for the Adipogenic Differentiation of Human Mesenchymal Stem Cells (hMSC)
This application note highlights tests to analyse the influence of cell culture surfaces on the growth and differentiation of hMSC. Two approaches were selected to analyse the adipogenesis of human mesenchymals stem cells: a high-content screening approach based on microscopic imaging and a high-throughput screening approach based on fluorescence measurements within a microplate reader.
Advanced TC™: An Innovative Surface Improving Cellular Assays
In this application note by Greiner Bio-One the novel Advanced TC™ surface is tested for its ability to facilitate serum-free cultivation of cells. In addition, the overall performance of cells in culture is assessed by microscopic and reader-based analysis of cell attachment, proliferation rates, transfection efficiency and a degree of differentiated cells, compared to a standard tissue culture surface.
