AdvanceBio Gly-X Technology
The Agilent AdvanceBio Gly-X (formerly ProZyme) technology is a new generation of N-glycan sample preparation
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Be ready for sample analysis faster with rapid (5-minute) enzymatic release of N-glycans and labeling with InstantDyes including the brightest in class InstantPC, or traditional reductive amination dyes. Innovative in-solution workflows combined with plate-based rapid cleanup enables sample prep in as little as 1 hour. Increase analytical sensitivity with a variety of dye options, while increasing sample throughput.
Dyes currently available for the Gly-X platform:
- InstantPC: a novel instant N-glycan label for liquid chromatography separations that provides markedly increased MS and HILIC-FLR sensitivity. Sample prep time: ~1 hour.
- 2-AB Express: 2-AB (2-aminobenzamide) has been used to generate labeled N-glycan data for over 20 years and is well established in many laboratories. Traditionally, labeling with 2-AB requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X 2-AB Express on-matrix labeling. Sample prep time: ~2 hours.
- InstantAB: ProZyme’s original InstantDye which allows room temperature, instant labeling of released N-glycans. Sample prep time: ~1 hour.
- APTS Express: APTS (8-aminopyrene-1,3,6-trisulfonic acid) is a negatively charged N-glycan label commonly used for capillary electrophoresis (CE) separations. Traditionally, labeling with APTS requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X APTS Express on-matrix labeling. Sample prep time: ~2.5 hours.
- InstantQ: a negatively charged N-glycan label for capillary electrophoresis (CE), designed for use with our Gly-Q N-glycan analysis platform. Sample prep time: ~1 hour.
N-Glycans profiling of MAbs EG2-hFc and Rituximab using the AdvanceBio Gly-X N-Glycan Prep with InstantPC kit
Download this application note to find a method for N-Glycan profiling. Protein glycosylation is one of the main sources of heterogeneity of therapeutic glycoproteins, with a potential impact on safety and efficacy, and is therefore considered a critical quality attribute (CQA). Therefore, this type of workflow holds great potential for reliable and reproducible sample preparation for high-throughput glycan profiling and quantitation. It can also easily be adapted to any analytical laboratory requirements.
High-throughput Human Serum and Immunoglobulin G N-Glycome profiling with the AdvanceBio Gly-X InstantPC Technology for biomarker discovery
In this application note, a method for high-throughput glycome profiling is described. Protein glycosylation is an intracellular process resulting in a glycan profile that is dependent upon the host cell and the translated protein. This workflow enables high-throughput efficiency and reproducibility in sample preparation for obtaining high-sensitivity N-glycome profiling and holds great potential for the discovery of disease diagnostic biomarkers.
A workflow for rapid sample preparation and exoglycosidase characterization of N-Glycans in antibodies
This application note describes use of the Agilent AdvanceBio Gly-X N-glycan prep with InstantPC kit, 96 ct (GX96-IPC) for high-throughput N-glycan profiling of Immunoglobulin G (IgG), a polyclonal antibody purified from human serum. This kit uses an innovative 96-well-plate-based workflow for rapid glycoprotein denaturation, deglycosylation, and instant glycan fluorescent derivatization.
Development of a rapid 2-AB sample preparation workflow for N-glycan release and labeling
In this application note, Agilent Technologies presents a comparison study consisting of two monoclonal antibodies (MabThera, NISTmAb) and one Fc fusion protein (Enbrel), using two different N-glycan sample preparation workflows it offers: 1) Agilent GlykoPrep Rapid N-Glycan Prep with 2-AB; 2) Agilent AdvanceBio Gly-X N-Glycan Prep with 2-AB Express (formerly ProZyme).
N-glycan analysis: Better together
In this brochure, Agilent Technologies introduces the Agilent and ProZyme sample preparation workflows that are designed to help push your glycoprotein analysis to new levels of performance.
