AdvanceBio Gly-X Technology by Agilent Technologies

Be ready for sample analysis faster with rapid (5-minute) enzymatic release of N-glycans and labeling with InstantDyes including the brightest in class InstantPC, or traditional reductive amination dyes. Innovative in-solution workflows combined with plate-based rapid cleanup enables sample prep in as little as 1 hour. Increase analytical sensitivity with a variety of dye options, while increasing sample throughput.

Dyes currently available for the Gly-X platform:

• InstantPC: a novel instant N-glycan label for liquid chromatography separations that provides markedly increased MS and HILIC-FLR sensitivity. Sample prep time: ~1 hour.
• 2-AB Express: 2-AB (2-aminobenzamide) has been used to generate labeled N-glycan data for over 20 years and is well established in many laboratories. Traditionally, labeling with 2-AB requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X 2-AB Express on-matrix labeling. Sample prep time: ~2 hours.
• InstantAB: ProZyme’s original InstantDye which allows room temperature, instant labeling of released N-glycans. Sample prep time: ~1 hour.
• APTS Express: APTS (8-aminopyrene-1,3,6-trisulfonic acid) is a negatively charged N-glycan label commonly used for capillary electrophoresis (CE) separations. Traditionally, labeling with APTS requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X APTS Express on-matrix labeling. Sample prep time: ~2.5 hours.  
• InstantQ: a negatively charged N-glycan label for capillary electrophoresis (CE), designed for use with our Gly-Q N-glycan analysis platform. Sample prep time: ~1 hour.