Trizma® Hydrochloride
Reagent grade, ≥99.0% (titration), crystalline
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Tris HCl, or Trizma® hydrochloride, is a widely utilized acidic buffer in the physiological pH range of 7.3 to 7.5. With a pKa of 8.08 at 25°C, it offers a pH range of 7.0 to 9.0, aligning with living organisms′ typical pH. Essential in various biological, cell biology, and biochemistry applications, Tris HCl is crucial for effective pH control in buffer solutions. Notably, Tris base and Tris hydrochloride alone lack sufficient buffering capacity, prompting their combination within the pH range of 7 to 9.
Tris buffers play a key role in techniques like protein electrophoresis and western blotting, serving in low ionic strength buffers for protein transfer. They are also integral to SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, especially in TAE and TBE buffers, are extensively employed in DNA agarose electrophoresis. These buffers are pivotal in diverse applications, including biochemistry, protein purification, and electrophoresis, showcasing the versatility and importance of Tris HCl in maintaining optimal pH for biochemical and cell biology research.
TechTalk: Buffer optimization for lateral flow assay development
Friday, June 19 at 16:00 BST | 17:00 CEST | 11:00 EDT | 08:00 PDT
Discover a practical approach to buffer selection and optimization for lateral flow assays (LFAs), with an emphasis on integrating buffer development early in the project.
In this 20-minute SelectScience® TechTalk we will cover antibody stability, non-specific binding, and kinetic considerations, as well as the impact of sample matrices and flow dynamics. We will also evaluate protein adsorption on membranes, detergent choice, and how optimized buffers for the sample and conjugate pads promote uniform flow and minimize nonspecific interactions.
The TechTalk will also cover production-scale buffer considerations and highlight Merck KGaA, Darmstadt, Germany’s LFA Development Services as a value-added resource. Attendees will gain actionable strategies to accelerate assay development, improve performance, and streamline scale-up.
This TechTalk is part seven of a seven-part expert-led TechTalk series on essential topics in LFA development. Register to watch live or on demand and receive exclusive invitations to upcoming sessions.
The Life Science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada.
TechTalk details
- Cost: Free to attend
- Location: Online
- Duration: 20 minutes
Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.
