Trizma® Base
BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
Receive your quote directly from the manufacturer.
Tris base, also known as Trometamol or Trizma base, plays a crucial role in diverse research applications as a biological buffer. Widely utilized in buffer formulations like TAE and TBE buffers, its pKa of 8.1 renders it well-suited for maintaining pH within the physiological range (pH 7.0-9.0) applicable to most living organisms. Researchers, however, need to be cautious when studying proteins, as Tris may potentially interfere with the activity of certain enzymes.
This compound can be employed independently as a buffer or as part of mixed buffer formulations, including Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, among others. Notably, Tris base is pure, essentially stable, and relatively non-hygroscopic.
In laboratory settings, Tris base proves indispensable for preparing buffers compatible with biological fluids, serving as a standard pH solution, and facilitating various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it aids in studies related to cell membrane permeability and buffer preparation.
TechTalk: Buffer optimization for lateral flow assay development
Friday, June 19 at 16:00 BST | 17:00 CEST | 11:00 EDT | 08:00 PDT
Discover a practical approach to buffer selection and optimization for lateral flow assays (LFAs), with an emphasis on integrating buffer development early in the project.
In this 20-minute SelectScience® TechTalk we will cover antibody stability, non-specific binding, and kinetic considerations, as well as the impact of sample matrices and flow dynamics. We will also evaluate protein adsorption on membranes, detergent choice, and how optimized buffers for the sample and conjugate pads promote uniform flow and minimize nonspecific interactions.
The TechTalk will also cover production-scale buffer considerations and highlight Merck KGaA, Darmstadt, Germany’s LFA Development Services as a value-added resource. Attendees will gain actionable strategies to accelerate assay development, improve performance, and streamline scale-up.
This TechTalk is part seven of a seven-part expert-led TechTalk series on essential topics in LFA development. Register to watch live or on demand and receive exclusive invitations to upcoming sessions.
The Life Science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada.
TechTalk details
- Cost: Free to attend
- Location: Online
- Duration: 20 minutes
Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.
