Tricine
≥99% (titration)
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Tricine is the most frequently used electrophoresis buffer. It is also useful for the resuspension of cell pellets. Tricine functions as a trailing ion and aids the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems. It has a lower negative charge than glycine, which helps it migrate faster. Tricine has a high ionic strength that allows increased ion movement and less protein movement. This in turn leads to the separation of low molecular weight proteins in lower percent acrylamide gels.
TechTalk: Buffer optimization for lateral flow assay development
Friday, June 19 at 16:00 BST | 17:00 CEST | 11:00 EDT | 08:00 PDT
Discover a practical approach to buffer selection and optimization for lateral flow assays (LFAs), with an emphasis on integrating buffer development early in the project.
In this 20-minute SelectScience® TechTalk we will cover antibody stability, non-specific binding, and kinetic considerations, as well as the impact of sample matrices and flow dynamics. We will also evaluate protein adsorption on membranes, detergent choice, and how optimized buffers for the sample and conjugate pads promote uniform flow and minimize nonspecific interactions.
The TechTalk will also cover production-scale buffer considerations and highlight Merck KGaA, Darmstadt, Germany’s LFA Development Services as a value-added resource. Attendees will gain actionable strategies to accelerate assay development, improve performance, and streamline scale-up.
This TechTalk is part seven of a seven-part expert-led TechTalk series on essential topics in LFA development. Register to watch live or on demand and receive exclusive invitations to upcoming sessions.
The Life Science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada.
TechTalk details
- Cost: Free to attend
- Location: Online
- Duration: 20 minutes
Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.
