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STEMdiff™ SMADi Neural Induction Kit

Serum-free medium kit for highly efficient SMAD inhibition-mediated neural induction of human ES and iPS cells

STEMCELL Technologies Inc.

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STEMdiff™ SMADi Neural Induction Kit consists of a defined, serum-free medium and supplement for the highly efficient neural induction of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. This kit combines STEMdiff™ Neural Induction Medium (Catalog #05835) with STEMdiff™ SMADi Neural Induction Supplement, which directs differentiation by blocking TGF-β/BMP-dependent SMAD signaling, resulting in efficient neural induction of even hard-to-differentiate cell lines. Neural progenitor cells (NPCs) can be generated using STEMdiff™ SMADi Neural Induction Kit with either an embryoid body (EB) protocol or monolayer culture protocol. The resulting cultures are enriched for central nervous system (CNS)-type NPCs, which express SOX1, Nestin, and PAX6. NPCs generated using this kit can be passaged as single cells and expanded in STEMdiff™ Neural Progenitor Medium (Catalog #05833). The NPCs can also be differentiated into neurons and glia using STEMdiff™ Neuron Differentiation Kit (Catalog #08500), STEMdiff™ Dopaminergic Neuron Differentiation Kit (Catalog #08520), or STEMdiff™ Astrocyte Differentiation Kit (Catalog #08540).

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