SPE-03
The SPE-03 8-Channel automated SPE system is an efficient, flexible and versatile workstation capable of processing up to 8 samples simultaneously

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PFAS free system and can be applied to different sample matrices for various analytes groups. User-friendly and preload
PFAS, mycotoxins in food, environment samples
This automated SPE system is easy to use and has different EPA methods re-loaded so that you can apply the methods without extra work. PFAS-free system.
Review Date: 29 Nov 2024 | PromoChrom Technologies
Time saver!!
PFAS 533 537.1
This automated system is great! Saves so much more time than using a manual manifold.
Review Date: 16 Jul 2024 | PromoChrom Technologies
Increased productivity with consistent results!
Emerging Contaminants in Drinking Water
Our laboratory purchased two Promochrom SPE-03 automated extractors to test for PFAS and 1,4 Dioxane. We previously have been performing the solid phase extractions manually. The SPE-03 has a compact footprint and is able to extract eight samples simultaneously. The SPE-03 is easy to set up and can be utilized for multiple applications. Methods are easy to set up with its touchscreen interface. The design of the automated extractor lends itself to easy maintenance when compared to some of their competitor's systems. We were able to achieve the proper method detection levels and have contaminant-free blanks. We have since purchased an additional two units and plan on ordering three more.
Review Date: 9 Feb 2022 | PromoChrom Technologies
Great instrument and customer service, saving time and effort.
PFAS analysis in environmental samples
We added a PromoChrom SPE-03 to our high-tech arsenal earlier this year to support our PFAS Analytical R&D. The SPE-03 performs automatic solid-phase extraction (SPE) which allows for consistency and reliability in sample extraction from batch to batch. In addition to saving hours of time for each batch, the instrument makes our analytical procedure for PFAS analysis easier and faster. The setup of SPE-03 is simple and intuitive. PromoChrom provides a video and a thorough manual for instrument installation and operation. PromoChrom is also very responsive when we have questions and need some help.
Review Date: 4 Aug 2021 | PromoChrom Technologies
Excellent product and customer service! 100% would recommend.
Extraction and analysis of water samples
The SPE-03 extraction systems has been a great tool. The user interface is extremely user friendly and easy to navigate. Method setup is very straightforward. Even with difficult sample matrices, the SPE-03 has provided extractions with much improved reproducibility over extractions using a vacuum manifold. PromoChrom has provided excellent after-sales care. Onsite setup and training was provided at no extra cost, although due to the simplicity of the system, setup and training could easily be provided remotely. They have always responded quickly to any questions or concerns, and have quickly shipped spare parts when needed. The SPE-03 system has become an invaluable asset, allowing a four fold increase in sample throughput, while also providing the previously mentioned reproducibility. I would highly recommend this extraction system to anyone.
Review Date: 20 Jul 2020 | PromoChrom Technologies
The SPE-03 provides impressive precision and automation for SPE applications.
Extraction of water samples for per/polyfluoroalkylated substances (PFAS)
The benefits of the SPE-03 for extraction of PFAS is the ability to extract 8 samples simultaneously, which is important for commercial laboratories where rapid sample throughput is an imperative. Similar systems on the market process samples in parallel, limiting productivity. The other benefit of the SPE-03 is the ability to rinse sample containers and use the rinsate to elute the SPE cartridges. This is a requirement of US EPA methods for PFAS (i.e. method 537) and other regulated procedures (e.g. US Department of Defence Quality Services Manual, Table B15). This ensures maximum recovery of PFAS adsorbed to the walls of sample containers. The two valve setup, with independent syringes per channel, prevents cross contamination between samples in the same process run. Method setup and operation is also straightforward using the touchscreen controller. Finally, controlled liquid dispensing handled by the syringed pumps ensures a high degree of precision relative to SPE performed manually by a laboratory technician.
Review Date: 5 Jul 2020 | PromoChrom Technologies
A fantastic instrument for target compounds like PFAS.
PFAS analysis in drinking water
Tiny footprint, easy to use with 8-channels for sample loading. Easy to modify the configuration to achieve desired extraction conditions.
Review Date: 19 Jun 2020 | PromoChrom Technologies
The SPE-03 8-Channel automated SPE system is an efficient, flexible and versatile automated SPE system capable of processing up to 8 samples simultaneously. Using PromoChrom's patented flow-path-integration technique, all 8 channels are controlled by just one multi-channel valve, making the design highly compact and simple.
The minimal-Teflon and automated sample bottle rinsing options make the system a popular choice for PFAS extraction and has been used for EPA Method 537.1/533, modifed methods and DoD QSM 5.3. Other applications include mycotoxins, pesticides, PAH, EPH, PCBs and protein purification.
Positive pressure is used to deliver fluids at fixed flow rates and prevent build up in SPE cartridges. All samples start and finish at the same time.
Key Features
- Processes up to 8 samples simultaneously in a single compact system
- Fully automated including sample bottle rinsing
- Minimal-Teflon option available for PFAS applications
- Positive-pressure fluid delivery to ensure consistent flow rate and prevent cartridge clogging.
- Intuitive built-in touch screen interface can store up to 100 methods, no need for external computer.
- Separate waste outlets for organic and aqueous waste
Applications
- PFAS
- Mycotoxins
- EPH
- PAH
- Pesticides
- PCBs
- Drugs
- Metabolites
- Protein
- Purification
Brochures
SPE-03: 8-Channel Automated SPE System
This product brochure contains information and technical specifications for the SPE-03 – a 8-channel automated SPE capable of cleaning 8 samples up to 4L at the same time.
Automating the Solid Phase Extraction of EPA Method 1633
Solid Phase Extraction (SPE) is employed by EPA Method 1633 for the extraction of PFAS across a range of matrices. PromoChrom’s automated SPE-03 system is a long-proven solution for PFAS and demonstrates high efficiency and quality of extraction for EPA Method 1633. Download this application note for an overview of PromoChrom's learnings, anti-clogging solutions, and significant time savings using the new Presto accelerated 8-Channel system. See how the newest Presto system offers significant boost in speed for larger volume samples and sensing capabilities to minimize disruptions when running challenging matrices.
Automated solid phase extraction using inline filters for PFAS in non-potable water following ISO 21675
In this application note, PromoChrom Technologies presents a streamlined extraction of non-potable water samples using PromoChrom’s high-capacity inline filters along with the automated SPE-03 system for the 30 compounds of ISO 21675, as well as 3 additional pertinent PFAS compounds.
Analysis of PFAS in drinking water using automated solid-phase extraction and LC/MS/MS following EPA method 533
This application note highlights the results of using PromoChrom’s fully automated 8-Channel solid phase extraction (SPE) system, SPE-03, to analyze PFAS in drinking water and other matrices, and draws comparison with manual extraction.
A unified solid phase extraction platform for disk and cartridge based extractions
Solid phase extraction of environmental samples typically involve either SPE cartridges or disks, depending on cost, availability, efficiency and sample matrix requirements. In this application note, PromoChrom Technologies introduces the SPE-03, a versatile system that works for a wide range of solid phase extraction applications.
Analysis of 1,4-dioxane in drinking water using automated solid phase extraction and GC/MS
In this application note, PromoChrom Technologies demonstrates the fully automated extraction of 1,4-dioxane in 500 mL of aqueous sample following EPA Method 522 Option 1. This note includes the background, precision (IDP), accuracy (IDA), and determination of detection limits (MDL).
Fully automated PFAS extraction for EPA Method 533
In this application note, PromoChrom Technologies demonstrates the full automation of EPA Method 533 cartridge extraction using SPE-03 and draws comparison with manual extraction.
Automation of PFAS analysis using EPA Method 537.1
In this application note, Promochrom Technologies explores key considerations for the automation of PFAS analysis using EPA Method 537.1 and outlines an effective protocol for the automation of SPE procedures using the SPE-03 8-channel SPE system.
Comparison of manual and automated SPE for analysis of Triazine
In this application note, Promochrom Technologies compares manual and automated SPE for analysis of triazine pesticides in tap water using the SPE-03 System.
A robust and simple method for solid phase extraction of PAH in water
In this application note, Promochrom Technologies outlines a simple, reliable and high-recovery method for automated extraction of PAH in water using just one solvent at 2.5 mL elution volume.
Automating solid phase extraction of PFAS
PromoChrom Technologies shares its experiences of working collaboratively with a number of PFAS laboratories over the years, and demonstrates how its SPE-03 system can automate PFAS extraction. Important considerations, challenges and approaches for achieving full automation of PFAS extraction are discussed for EPA Methods 537.1/533, ISO 21675, draft EPA Method 1633 and a proprietary soil extract clean up method.
8-channel automated protein purification system
In this video, PromoChrom Technologies describes its SPE-03, which promises to maximize your protein purification throughput with this compact and flexible system. Compatible with a range of affinity, ion exchange, hydrophobic interaction columns, the SPE-03 can be used for antibodies, antigens, oligonucleotides, enzymes, metabolites, and more.
Automated SPE for PFAS using EPA Draft Method 1633 (Extraction of non-potable PFAS matrices)
The SPE-03 8-Channel system, best known for automating PFAS extraction following EPA Methods 537.1 and 533 is now being used for EPA draft method 1633. This video touches on many considerations of the method and how the SPE-03 with MOD-00P Volume-Matrix Plus configuration can address each solid-phase extraction requirement for non-potable matrices. Full automation of the method is achieved through automatic rinsing of sample containers of all sizes mentioned in draft Method 1633, allowing the SPE procedure to run unsupervised.
Anti-clogging frits to alleviate SPE cartridge clogging and blockage
PromoChrom has developed an anti-clogging frit - a simple yet effective multi-layer frit to alleviate the effects of clogging. Whether you use automated SPE or Vacuum Manifold, PromoChrom's anti-clogging frits can help achieve smoother extraction of challenging samples. Watch the video below to find out how you can be more efficient in your solid phase extraction experiments.
Fully automated solid phase extraction for PFAS with PromoChrom's SPE-03
In this video, PromoChrom Technologies demonstrates how the SPE-03 from PromoChrom can perform a fully automated and validated solution for PFAS extraction.
Volume-matrix plus configuration for the SPE-03 automated 8-channel SPE system
In this video, PromoChrom Technologies demonstrates how the SPE-03 system promises to perform easy and efficient loading of large volume samples, handling of sample particulates, and automatic bottle rinsing.
Speed up sample preparation with 8-channel SPE
Looking for an 8-channel SPE system to speed up sample preparation? The SPE-03 is designed for high-volume automated solid phase extraction of water, food, soil and biological samples, and can process eight samples up to 4L in parallel.
Fully automated SPE for PFAS extraction
In this video, PromoChrom Technologies highlights the advantages of the SPE-03 8-channel system for fully automated and efficient PFAS extraction. This system has been validated by customers for PFAS in drinking and potable water following EPA Method 537, EPA Method 537.1, EPA Method 533, DoD and modified methods.
Perfecting automated solid phase extraction for PFAS and other perfluorinated compounds in drinking water
Learn how one U.S. analytical lab has struck a partnership with its technology provider to become an industry leader in drinking water analysis




































