sciFLEXARRAYER S100

Diagnostics of infectious diseases based on carbohydrate antigens is a sensitive alternative to commonly used capturing protein agents on lateral flow test strips. This application note describes a rapid test technology for 13 major disease-causing bacteria strains from Streptococcus pneumoniae, Neisseria meningitides and Haemophilus influenzae using polysaccharides on lateral flow membranes.

An essential component of today's research in life science is the use of automated liquid handling equipment. The transferred volumes are becoming increasingly small down to nano- and picoliter range. Verifying the absolute transferred volume and the well-to-well reproducibility is important to ensure the quality of the process but there are only a few methods available that can quickly and accurately measure dispensed volumes in this low range. The results presented in this application note show outstanding accuracy and reproducibility of the sciFLEXARRAYER in dispensing of volumes ranging from 5 nl to 1000 nl.

Miniaturization and multiplexing of cell culture on biochip is attracting a growing interest with the development of single-cell assays and cell-based biosensors. Here, our collaborators report rapid methods for microwell preparation and controlled dispensing of different cell lines within these fabricated microwells.

sciPOLY3D opens the door to immobilize DNA probes without a functional group directly to an unmodified polymer support and acts as a surface-attached hydrogel, where probe oligonucleotides are embedded covalently. Thus anchorage of the probes to the surface is on the one hand very robust and withstands very stringent washing procedures or even elevated temperatures up to 100 °C. This feature renders it suitable for chipbased PCR and melting curve applications.

Most conventional immobilization strategies for proteins rely on adsorptive immobilization only or surface functionalization of the whole substrate with e.g. carboxy or epoxy moieties. sciPOLY3D proteins by SCIENION AG can be covalently attached to virtually all polymers without pre-treatment, simplifying and speeding up whole process of microarray printing and allowing to be integrated into a high-throughput production line.

Carbohydrate specific antibodies play an important role in human disease, meaning that carbohydrate assays are highly desirable for diagnostic purposes. This application note presents a study in which a selection of glycans were printed on glass slides using a sciFLEXARRAYER and detected using both a colorimetric and fluorescent based method. It was found that both methods reliably detected the binding of anti-carbohydrate antibodies from human serum to the microarray.

Multiyear alliance underpins Randox commitment to increase biochip manufacturing to transform rapid diagnostic testing