RediSep ® Gold ® Reversed Phase C18

This application demonstrates how the CombiFlash® EZ Prep system with PurIon Model L mass spectrometer and RediSep® Prep columns allow research labs to save time and money by purifying crude peptides received from outside synthesis labs themselves. By purifying peptides as needed, a continuous supply of fresh peptide is always available as peptides can degrade over time during storage.

Carbohydrate compounds are synthesized for a variety of purposes; they are used as probes in life sciences such as mechanism studies. This application note provides a summary of methods for purifying compounds containing carbohydrate moieties and detection/fractionation of these compounds. Synthesized compounds and natural products are examined with examples from the published literature using CombiFlash® systems or RediSep® columns from Teledyne Isco.

Ethyl acetate is one of the most commonly used solvents in flash chromatography. It is readily available and easy to remove by rotary evaporation. However, ethyl acetate adsorbs in the wavelength range of 200 to 235 nm, a region where many compounds also absorb UV light. In this application note, acetone is presented as an alternative to ethyl acetate for these compounds. Acetone is slightly more polar than ethyl acetate allowing for faster elution of some compounds.

This application note describes a generalized approach to separating peptide mixtures into fractions on reusable reversed phase RediSep® C18 columns from Teledyne Isco. Peptide mixtures may be separated into individual peptide species. If the separate and more specific peptides can be contained in a single fraction, this can greatly expedite many scientific processes.

In this application note discover how proper preconditioning, cleaning, and storage of reusable Teledyne Isco RediSep® Rf reversed phase C18 and RediSep Rf Gold® high performance C18 columns will help to extend column life and improve column consistency and reliability.

Natural sources contain a large number of compounds that range from very non-polar materials, such as terpenes, to highly polar compounds such as tannins. Wide Polarity Range Chromatography is a method to resolve complex mixtures into various groups of molecules that are easier to purify in subsequent steps. This poster describes columns suitable for use with Wide Polarity Range Chromatography including the RediSep Gold® C18, diol, and silica and the RediSep® alumina columns.

Samples are often dissolved in non-volatile solvents, such as DMF and DMSO, for experiments such as NMR studies. Recovery of the sample from these solvents is difficult because of the solvent’s high boiling point. In this application note, the RediSep Rf Gold® C18Aq columns are used to retain the desired compound while removing the non-volatile solvent. The compound is then released using a solvent with a lower boiling point that is more easily evaporated. This method allows the recovery of valuable material for further experiments.

Compounds are often purified with solvents that contain salts or buffers that need to be removed prior to subsequent studies with the compound. In this application note, the RediSep Rf Gold® C18Aq columns are used to adsorb compounds under highly aqueous conditions for removing salt from samples. The method described also removes a large portion of water from samples dissolved in aqueous solvents facilitating evaporation. The procedure is similar to solid phase extraction, but on a larger prep scale.

The entire class of cannabimimetic compounds has been made illegal in the United States, which requires the generation of reference compounds for all cannabimimetic compounds. In this application note, Teledyne Isco CombiFlash® systems and RediSep Rf Gold® columns are used to easily purify both synthetic and natural cannabinoid compounds from seized samples.

Compounds are often purified with solvents that contain salts or buffers that need to be removed prior to subsequent studies with the compound. The RediSep Rf Gold® C18Aq columns have the ability to adsorb compounds under highly aqueous conditions and are ideal for removing the salt from a sample. The method described also removes a large portion of water from samples dissolved in aqueous solvents facilitating evaporation. The procedure is similar to solid phase extraction, but on a larger prep scale.