RediSep ® Gold ® Amine
High performance normal- or reversed-phase purification of compounds with basic properties.
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RediSep Rf Gold Amine columns can be used in either normal or reversed phase for the purification of compounds with basic properties.
Purification on high-performance amine-functionalized silica eliminates the need to add a mobile phase modifier such as triethylamine as it is often done for purification on normal phase silica, and thus reduces the time required to remove solvent from purified fractions. This smaller, spherical media outperforms standard Flash-grade Amine media.
RediSep® Rf Gold® Amine Features:
- High flow rates for fast resolution of target compounds.
- Column sizes available for purifying milligrams to several grams.
- RFID technology for automatic set up on compatible instruments.
- Fits all Isco CombiFlash automated organic purification instruments.
- Long shelf life in airtight, resealable packages.
- Luer lock end fittings.
Save Time and Money by Purifying Peptides Yourself
This application demonstrates how the CombiFlash® EZ Prep system with PurIon Model L mass spectrometer and RediSep® Prep columns allow research labs to save time and money by purifying crude peptides received from outside synthesis labs themselves. By purifying peptides as needed, a continuous supply of fresh peptide is always available as peptides can degrade over time during storage.
Strategies to Purify Carbohydrate Based Compounds with Flash Chromatography
Carbohydrate compounds are synthesized for a variety of purposes; they are used as probes in life sciences such as mechanism studies. This application note provides a summary of methods for purifying compounds containing carbohydrate moieties and detection/fractionation of these compounds. Synthesized compounds and natural products are examined with examples from the published literature using CombiFlash® systems or RediSep® columns from Teledyne Isco.
Use of RediSep Gold® Amine Columns in the Weak Ion Exchange Mode
The RediSep Rf Gold® Amine columns are useful for normal phase purifications, but can also be converted for use as a weak anion exchange (WAX) column. This application note describes the conversion of the amine column to a WAX column and an example run with the converted column. Washing and conditioning the column for another run is also described.
Wide Polarity Range Flash Purifications
Natural sources contain a large number of compounds that range from very non-polar materials, such as terpenes, to highly polar compounds such as tannins. Wide Polarity Range Chromatography is a method to resolve complex mixtures into various groups of molecules that are easier to purify in subsequent steps. This poster describes columns suitable for use with Wide Polarity Range Chromatography including the RediSep Gold® C18, diol, and silica and the RediSep® alumina columns.
