RASTRUM Allegro
RASTRUM™ Allegro is a revolutionary 3D cell culture platform designed to generate biologically relevant models at scale. It streamlines the creation of complex, structured 3D cell environments, accelerating drug discovery and disease research.
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RASTRUM™ Allegro is transforming 3D cell culture by enabling researchers to generate precise, reproducible 3D models with unparalleled consistency. Traditional methods for creating 3D cell cultures can be manual, variable, and difficult to scale—RASTRUM Allegro eliminates these challenges by standardizing the deposition of cells and extracellular matrices to build biologically relevant microenvironments with ease.
By leveraging drop-on-demand technology, the platform precisely controls matrix composition and cell placement, ensuring models that better reflect in vivo-like conditions. The result? High-throughput, low-variability 3D cultures that improve predictive power for drug discovery, disease modeling, and translational research. With intra- and inter-plate variation below 10%, RASTRUM Allegro delivers high-quality, reproducible data, critical for preclinical research.
With support for multiple cell types, co-culture systems, and tunable extracellular matrix compositions, RASTRUM Allegro allows scientists to develop phenotypically relevant models across a range of applications, including oncology, neuroscience, and immuno-oncology.
Key Features:
- Scalable 3D cell culture platform for high-throughput studies
- Drop-on-demand technology for precise matrix and cell deposition
- Generates biologically relevant 3D models with high reproducibility
- Intra- and inter-plate variation below 10% for robust, reliable results
- Supports co-culture systems and multiple extracellular matrix compositions
- Designed for drug discovery, disease modeling, and translational research
Applications:
- Oncology: Development of patient-derived tumor models for drug response testing
- Neuroscience: 3D neural cultures for studying neurodegeneration and synaptic function
- Immuno-oncology: Immune-competent tumor models to assess T-cell and checkpoint inhibitor interactions
- Cell therapy & regenerative medicine: 3D microenvironments for stem cell research and tissue engineering
Scalability and reproducibility for high-throughput biological modeling in 3D cell culture
The RASTRUM™ Allegro platform provides a powerful solution for generating consistent 3D models at scale. While increasingly it is understood that 3D biology can offer insights that 2D monolayers cannot, challenges remain in the development of 3D cell/tissue models. Factors such as cell source, matrix choice, and experimental handling can vary widely and ultimately impact reproducibility. For applications that demand large datasets, such as drug discovery, reproducibility is paramount. The coefficient of variation (CV) offers a standardized measure to evaluate the consistency of data. Whilst the acceptance criteria for CV values can depend on assay type, for multiwell-plate–based biological assays these CV values have typically been defined as 10–30%. Furthermore, CV values less than 10% have been considered as excellent and 10-20% as good in a variety of biological contexts.
Here, Inventia demonstrate that the RASTRUM Allegro is capable of delivering CV values that are reproducible across model architectures and cell types, and suitable for evaluation of various downstream applications. Post-print CVs were consistently <10% across various architectures and plate formats. Over a range of downstream biological assays, it has achieved CVs ranging from 7.1% (96-well drug-dosing viability assay) up to 18.6% (384-well drug-dosing viability assay).
