Products & ReviewLife Sciences

QuantiFluor® ONE dsDNA Dye

Promega Corp.E4891

The QuantiFluor® ONE dsDNA Dye is a fluorescent double-stranded DNA-binding dye (504nmEx/531nmEm) prepared for an “add-and-read” format making sample quantitation easy. This dye enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA). The QuantiFluor® ONE dsDNA Dye is intended for use as a general purpose reagent in the US and is suitable for use by clinical laboratories as a component of la…

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Description

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The QuantiFluor® ONE dsDNA Dye is a fluorescent double-stranded DNA-binding dye (504nm_Ex/531nm_Em) prepared for an “add-and-read” format making sample quantitation easy. This dye enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA).

The QuantiFluor® ONE dsDNA Dye is intended for use as a general purpose reagent in the US and is suitable for use by clinical laboratories as a component of laboratory developed tests. Outside of the US, this product is intended for research use only.

The QuantiFluor® ONE dsDNA Dye can be used with any fluorometer that is capable of measuring fluorescence at the appropriate excitation and emission wavelengths.

QuantiFluor® ONE dsDNA Dye Features & Benefits:

Easy to Use - Add-and-read format makes this dye simple to use—no dilutions, no extra tubes.
Sensitive - Significantly better sensitivity compared to absorbance at 260nm (NanoDrop®), allowing users to quantitate their low-concentration samples with confidence.
Highly Specific to dsDNA - Minimal binding to ssDNA, RNA, protein and interfering compounds.
Flexible with Instrumentation - Compatible with any fluorometer capable of measuring the appropriate fluorescence excitation and emission spectra.

Applications:

dsDNA quantitation.

Application NoteLife Sciences

Purification of Amplifiable Nucleic Acid from Oil Palm and Rice Leaves and Seeds Using the Maxwell® 16 LEV Plant DNA Kit

This poster evaluates a novel cellulose-based paramagnetic particle technology to purify nucleic acid (NA) from Elaeis guineensis (oil palm) and Oryza sativa (rice) plant leaf and seed lysates in an automated format using the Maxwell® 16 System. Plant NA purification provides a variety of unique challenges due to the broad diversity of plants, their extracellular structures, and endogenous compounds (e.g. polysaccharides, phenolics) that can co-purify with NA and inhibit downstream enzymatic assays. Extracted NA were evaluated for yield and amplifiability in qPCR and RT-qPCR, and the data collected supports the broad utility of the novel cellulose-based technology and Maxwell® 16 System for automated plant NA purification.

12 Jan 2016

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QuantiFluor® ONE dsDNA Dye - Promega Corp. - Life Sciences

QuantiFluor® ONE dsDNA Dye

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