Opentrons Flex Robot

Measuring protein concentrations is an essential part of many routine lab procedures as it is often a prerequisite before subjecting samples to electrophoresis, chromatography, or other molecular biology analysis. Using a robotic liquid handler allows the lab not only to process higher throughput numbers of samples with minimal hands-on time, but also to establish a standardized protocol to consistently generate reliable test results in the long run. Explore three Opentrons Flex™ protocols that have been developed to respectively perform common protein quantification methods: BCA protein assays, Bradford protein assays and BCA peptide quantification assays.

As the ambition of sequencing projects and capacities of sequencing platforms grows, labs are increasingly looking for ways to streamline laborious and time-consuming preparation of libraries. One way of achieving this is through automating the library prep workflow. Additionally, library prep workflows are becoming more efficient as a result of advancements in library prep chemistry, with features that support automation, shorter workflows and/or fewer pipetting steps. Explore the automation of QIAGEN’s QIAseq FX and Normalizer chemistries on the Opentrons Flex™ for efficient preparation of E. coli, human genomic and mixed microbial DNA libraries.

Opentrons demonstrates automation of three popular life science workflows (nucleic acid extraction, NGS library prep and small-scale protein purification) on a new automation platform, the Opentrons Flex™. See how highly accurate results are gained on a system that is flexible and easy to implement for many different types of life science laboratories.

Efficiently isolating genomic DNA from cells and tissues is a cornerstone in life science and applied testing labs. Explore the automated purification of genomic DNA from HeLa cells using the Opentrons Flex™ Nucleic Acid Extraction Workstation, featuring the Flex™ Magnetic Block, Opentrons Heater-Shaker, and Flex™ 1- and 8-Channel Pipettes (5–1000 μL). The described application is adaptable for various sample materials, including DNA extraction from alternative eukaryotic cells or diverse tissue biopsy samples.

Wednesday, March 5, 2025 at 17:00 GMT / 18:00 CET / 12:00 EST / 09:00 PST

Single-cell RNA sequencing (scRNA-seq) is a powerful technique for teasing apart the heterogeneity between cells in a population and identifying subtypes within a sample.

Parse Biosciences’ Evercode™ technology leverages the proprietary split-pool combinatorial barcoding to uniquely label individual cells, enabling scalable and high-quality single cell data.

This webinar will demonstrate how the whole workflow, from fixation to library preparation, can be automated efficiently on the Opentrons Flex. This webinar will also present data that shows high-quality scRNA-seq libraries generated from peripheral blood mononuclear cells (PBMCs).

Key learning objectives

• Gain a deeper understanding of Parse Biosciences’ Evercode™ technology and its ability to provide scalable, high-quality scRNA-seq data through split-pool combinatorial barcoding
• Understand how you can leverage the Opentrons Flex liquid handling platform to automate popular genomics workflows, including scRNA-seq
• Learn how automating the entire Evercode WT workflow on Opentrons Flex can reduce needed hands-on time by over 10 hours for a single experimental run
• Review data from scRNA-seq libraries of PBMCs created using an automated sample prep workflow

Who should attend?

• Scientists in academia, core labs, and pharma and biotech who are leveraging transcriptomics techniques
• Anyone interested in optimizing their scRNA-seq sample prep workflows

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.