MegaMix
MegaMix: All-in-one PCR solution with Taq DNA polymerase, 220 μM dNTP, and 2.75 mM MgCl2 in Microzone’s enhancing buffer for swift, reliable, and high-yield amplification.
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MegaMix contains all the components needed to perform PCR swiftly and reliably. The 1.1X mix contains standard Taq DNA polymerase, 220 μM dNTP and 2.75 mM MgCl₂ in Microzone’s proprietary enhancing buffer.
MegaMix is optimized to provide high yields under standard conditions.
Key Features
- More confidence in amplification and PCR tests.
- Mastermix format is ready to use or as a 2x concentration.
- Both single and double concentrations. Get the right mix for your tests quickly and easily.
- Easy setup and PCR optimisation.
- Broad range of templates and conditions.
- Extremely stable—can be freeze-thawed many times.
Applications
- Routine PCR up to 6kb.
- Genotyping.
- TA cloning.
- Microarray Analysis.
- Colony PCR.
- Amplification of GC and AT rich templates.
- Methylated DNA.
Preparation of bacterial samples for sequencing
It is estimated that hospital-associated infections (HAI) cost between $35 and $45 billion in the USA and the NHS £3 billion in the UK, as well as countless lives. To counter such costs, Microzone proposes a method for the rapid preparation of cultured bacterial samples for Sanger Sequencing. Utilization of Microzone's 'free from' technology is highlighted for allowing for fast, efficient workflows only requiring a centrifugation step and not requiring automation.
