Macrophage Base Medium XF
The PromoCell Macrophage Generation Media were developed for easy and efficient generation of the full spectrum of M1- and M2-polarized macrophages directly from freshly isolated peripheral blood monocytes.
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The PromoCell Macrophage Generation Media were developed for easy and efficient generation of the full spectrum of M1- and M2-polarized macrophages directly from freshly isolated peripheral blood monocytes.
The PromoCell M1-Macrophage Generation Medium xf contains GM-CSF and allows for the generation of M1-polarized macrophages, whereas the M2-Macrophage Generation Medium xf contains M-CSF and produces M2-polarized macrophages. Both media are ready-to-use and tested in-house for successful differentiation of M1-/M2-macrophages via flow cytometry.
The Macrophage Base Medium xf is the user-customizable version of the Macrophage Generation Media xf and does not include cytokines. It comes without cytokines as a universally applicable MDM culture system featuring a fully user-customizable macrophage differentiation and activation process.
The Monocyte Attachment Medium allows for the efficient adherence of fresh monocytes as well as for adherence selection of monocytes from freshly isolated Mononuclear Cells (MNC) while maintaining optimal cell health. Thus, the time-consuming and costly immunomagnetic purification of monocytes prior to macrophage differentiation is not necessary.
All PromoCell Macrophage Generation Media xf are defined and xeno-free media formulations, free of all animal-derived components and substances of human origin with Human Serum Albumin as the only exception.
Macrophage Generation Media xf Advantages:
- Easy access to the full range of macrophage activation states
- Obtain fully functional, untouched macrophages directly from peripheral blood mononuclear cells
- Generate macrophages from up to 5 buffy coats per medium bottle within one week
- No time-consuming and costly immunomagnetic purification of monocytes necessary
- No immunogenic or deleterious effects from animal-derived components
PromoCell's macrophage cell culture portfolio
In this flyer, PromoCell presents its full range macrophage cell culture range, which includes media systems for generation of macrophages from both peripheral blood mononuclear cells (PBMCs) and tumor-associated macrophages (TAM).
Standardized Culture of Established Cancer Cell Lines under Serum-Free and Xeno-Free Conditions
The PromoCell Cancer Cell Line Medium XF was designed with the aim of achieving a universal, consistent and xeno-free environment for culturing most of the commonly used human cancer cell lines. Being broadly usable across these common adherently growing cancer cell lines, this new culture medium is a cost-effective solution for ensuring efficient, genuinely standardized routine cultures.
Large-Scale Differentiation of Fully Customized Monocyte-Derived Macrophages from Human PBMCs In Vitro
Learn a method for easy and cost-efficient differentiation of pure monocyte-derived macrophages directly from peripheral blood mononuclear cells (PBMCs) as a starting material, using PromoCell's Macrophage Base Medium DXF in combination with the Monocyte Attachment Medium.
Macrophage Polarization Plasticity in Health and Disease
Macrophages are tissue-resident professional phagocytes and antigen-presenting cells (APC), that form as a result of the differentiation of circulating peripheral blood monocytes. They perform acrive and regulatory immune functions in both innate and adaptive immunity. Macrophages influence the outcome of many diseases, e.g. allergic and autoimmune disorders, cancer, diabetes, atherosclerosis, rheumatoid arthritis and metabolic syndrome. It is difficult to isolate sufficient amounts of primary human macrophages of a homogeneous phenotype, and they do not proliferate in culture. The cells obtained often exhibit significant phenotypical heterogeneity. Human monocyte-derived macrophages (hMDM) are highly pure and readily available, can be differentiated into macrophages in vitro and are therefore an excellent alternative.
Differentiation of M1- or M2- Macrophages from PBMC/Monocytes
Macrophages are tissue-resident professional phagocytes and antigen-presenting cells (APC), which differentiate from circulating peripheral blood monocytes. They perform important active and regulatory functions in innate as well as adaptive immunity. The PromoCell Macrophage Generation Media were designed for the straightforward and efficient differentiation of highly pure M1- or M2-macrophages directly from PBMC as a starting material. Included in this application note is a protocol overview using PromoCell M1-/M2-Macrophage Generation Medium DXF.
Macrophage Subtypes: Polarization, activation and plasticity
Macrophages are multitasking cells at the first line of defense against pathogen invasion. In this video, discover how they can also play a major role in destroying tissues, developing cancer, an autoimmune disorder, or allergies.
Immuno-oncology advances: From tumor microenvironments and CAR-T cell workflows to macrophage generation
Discover the latest technologies and techniques that are revolutionizing immuno-oncology research
