LV200 - LuminoView bioluminescence microscope system
Bioluminescence imaging system
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Quantitative gene expression, promotor studies and Ca2+ imaging without phototoxicity and autofluorescence.
The detection of bioluminescent markers like Luciferase, Aequorin or BRET samples was limited to luminometers or low resolution imaging. The high signal to noise ratio and the lack of autofluorescence are just a few reasons for making the application of bioluminescent markers popular in HTS assays and in vivo imaging of small laboratory animals. Because signal intensity dramatically drops with increasing magnification there was no way of using the advantages of bioluminescence for microscopy in the past. This will change with the new and unique luminescence microscope LuminoView LV200 introduced by Olympus.
Optics specifically designed for luminescence increase the sensitivity of light detection remarkably and enable high resolution imaging of cells and tissues with standard CCD and EM-CCD cameras. This outstanding innovation will backup standard fluorescence microscopy when phototoxic effects and strong autofluorescence exclude the application of excitation light for imaging. Main applications especially when light sensitive cells are involved are promotor studies, time lapse monitoring of gene expression, Ca2+ imaging and single cell ATP measurements.
Bioluminescence Microscopy for Cellular Level Circadian Analysis in the Suprachiasmatic Nucleus (SCN)
Cellular level analysis of variations in the SCN has enabled new research into circadian rhythms and brain functionality. In this application note a new technology using optimized bioluminescence is described. Consistent cellular-level clarity is achieved by optimizing the Olympus LV200 Luminoview, offering the benefit of advanced imaging capabilities for long-term live cell imaging.
Development of the Circadian Oscillator during differentiation of ES Cells
A method is presented by Olympus Europa Holding GmbH to clarify the process of the circadian clock formation using ES and iPS cells at the single cell level using a luminescence imaging system. The report indicates that formation of the circadian clock oscillator is closely related to cell differentiation. Monitoring clock gene expression
allows the observation of an intrinsic program which controls the formation of the circadian oscillator during the differentiation process of ES cells in vitro.
