Ionic® Purification System

Formalin-fixed paraffin-embedded (FFPE) tissue preservation is a method that was first developed 125 years ago to maintain tissue morphology. With the advent of DNA sequencing and other modern molecular methods in the past few decades, there has been great demand to extract DNA and RNA from FFPE tissue. However, FFPE is an imperfect sample type for such analyses because the nucleic acids are often highly degraded and cross-linked to proteins, as a result of the fixation process. Nevertheless, the dominance of FFPE samples in archives and its use in prospective studies means FFPE is likely to be widely used for years to come.

This application eBook provides an expert guide on how to improve and optimize DNA and RNA extraction from FFPE tissue, outlines isotachophoresis, explores new methods to enhance nucleic acid extraction and purification, and provides valuable information on how to effectively identify novel biomarkers from FFPE samples.

This eBook will also cover:

• New technologies and solutions designed to enhance nucleic acid extraction and purification
• Methods to generate higher yields with simple and automated workflows

To compare the performance of the Purigen Ionic system, DNA from 32 FFPE tissue blocks was purified with both the Ionic system and a commercially available column-based purification kit for FFPE samples. In this poster, Purigen assessed the quantity and quality of the purified DNA with the Qiagen MRef multicopy reference assay and the Agilent Technologies QC Plex Assay.

Simultaneous extraction and purification of both RNA and DNA from FFPE tissue samples is critical to enable multiple downstream analyses from rare or limited samples. To avoid yield loss, most technologies use a serial isolation approach where DNA and RNA are separated after lysis by filtration or precipitation before entering separate purification workflows. This results in twice the number of purification steps per sample. With simultaneous extraction and purification of RNA and DNA from FFPE tissue samples without yield loss, the Ionic® FFPE Complete Purification Kit for the Ionic® Purification System offers a significant savings on total workflow time and reduces hands-on time by more than 75%.

In this application note, to compare the efficiency of DNA extraction and purification from FFPE samples, DNA was purified from a single 10 µm thick section from eight FFPE tissue blocks (18 years of age) containing breast, colon, or lung tissue using the Purigen Ionic® Purification System and a commercially available column-based kit. Adjacent scrolls were harvested from each block and processed separately by each method. The DNA purified by each method was quantified prior to NGS.

The revolutionary Ionic® Purification System from Purigen Biosystems uses isotachophoresis to extract, purify, and concentrate nucleic acid (perform DNA extraction) from biological samples. Nucleic acids remain in their native form, not denatured or dehydrated, and there’s also no binding to, or stripping from, fixed surfaces. Get pure, abundant, and ready-to-use nucleic acid in 60 minutes.

ABRF 2022 Technology Showcase with special guest speaker Dr. Jian Gu from the University of Texas, MD Anderson Cancer Center. This video discusses how the next-gen Ionic® Purification System enables pure nucleic acid extraction from FFPE samples using our proprietary charge-based isotachophoresis (ITP) technology.

In this video, Purigen discusses how the next-gen Ionic® Purification System enables pure nucleic acid extraction from FFPE samples using proprietary charge-based isotachophoresis (ITP) technology.

FFPE tissue samples with a low abundance of nucleic acid present challenges for cancer genomics as they can produce insufficient DNA or RNA for downstream analyses. In this video, Purigen Biosystems review the data that demonstrates the ability of the Ionic® Purification System to recover sufficient yields of high-quality DNA, RNA, or miRNA from FFPE tissue samples.

Researchers and clinicians continue to be challenged by FFPE tissue samples with a low abundance of high-quality nucleic acid. FFPE samples frequently fail to produce DNA or RNA with sufficient yield or quality for downstream analyses when conventional extraction methods are used. Despite efforts to optimize these labor-intensive extraction methods, extracts from FFPE samples frequently fail to qualify for next-generation sequencing or fail to yield actionable results when sequenced. In this webinar, we will review data from samples purified on the Ionic® Purification System that demonstrates the ability of the system to recover DNA, RNA, or miRNA from challenging FFPE tissue samples with sufficient yield and quality for downstream molecular analyses.

The new Ionic® FFPE Complete Purification Kit from Purigen Biosystems provides simultaneous purification of DNA and RNA, including miRNA, from FFPE tissues. While most technologies use a serial approach to separately isolate DNA and RNA, this new kit enables a single, automated workflow using the Ionic® Purification System that requires 75% less hands on time.

Download this eBook to discover tips, tricks, and tools to improve your FFPE DNA and RNA extraction

New automated Ionic Tissue to Pure DNA Kit promises to recover more than three times the amount of high-quality nucleic acid from limited samples compared to leading column-based products

Company rapidly expanding commercial operations to enable more researchers to extract higher yields of DNA and RNA from FFPE samples in one hour with minimal hands-on time