InstantPC Labeled Glycans
Agilent InstantPC (formerly ProZyme) labeled glycan standards and libraries are commonly used as qualitative standards for LC/FLD and LC/MS.
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Agilent InstantPC (formerly ProZyme) labeled glycan standards and libraries are commonly used as qualitative standards for LC/FLD and LC/MS. These standards come pre-labeled with InstantPC, ready to reconstitute and inject. Better detection of low abundance N-glycans in your LC/FLD or LC/MS method translate to higher method sensitivity.
Features:
- InstantPC labeled glycan standards are commonly used as qualitative standards for LC/FLD and LC/MS
N-Glycans profiling of MAbs EG2-hFc and Rituximab using the AdvanceBio Gly-X N-Glycan Prep with InstantPC kit
Download this application note to find a method for N-Glycan profiling. Protein glycosylation is one of the main sources of heterogeneity of therapeutic glycoproteins, with a potential impact on safety and efficacy, and is therefore considered a critical quality attribute (CQA). Therefore, this type of workflow holds great potential for reliable and reproducible sample preparation for high-throughput glycan profiling and quantitation. It can also easily be adapted to any analytical laboratory requirements.
High-throughput Human Serum and Immunoglobulin G N-Glycome profiling with the AdvanceBio Gly-X InstantPC Technology for biomarker discovery
In this application note, a method for high-throughput glycome profiling is described. Protein glycosylation is an intracellular process resulting in a glycan profile that is dependent upon the host cell and the translated protein. This workflow enables high-throughput efficiency and reproducibility in sample preparation for obtaining high-sensitivity N-glycome profiling and holds great potential for the discovery of disease diagnostic biomarkers.
A workflow for rapid sample preparation and exoglycosidase characterization of N-Glycans in antibodies
This application note describes use of the Agilent AdvanceBio Gly-X N-glycan prep with InstantPC kit, 96 ct (GX96-IPC) for high-throughput N-glycan profiling of Immunoglobulin G (IgG), a polyclonal antibody purified from human serum. This kit uses an innovative 96-well-plate-based workflow for rapid glycoprotein denaturation, deglycosylation, and instant glycan fluorescent derivatization.
Development of a rapid 2-AB sample preparation workflow for N-glycan release and labeling
In this application note, Agilent Technologies presents a comparison study consisting of two monoclonal antibodies (MabThera, NISTmAb) and one Fc fusion protein (Enbrel), using two different N-glycan sample preparation workflows it offers: 1) Agilent GlykoPrep Rapid N-Glycan Prep with 2-AB; 2) Agilent AdvanceBio Gly-X N-Glycan Prep with 2-AB Express (formerly ProZyme).
