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Incucyte® Mouse IgG1 FabFluor-488 Antibody Labeling Reagent for Live-Cell Immunocytochemistry

Sartorius Group4745Available: Worldwide

One vial (50 µg) of lyophilized FabFluor-488 conjugate and one vial (200 µL) of IncuCyte® Opti-Green background suppressor sufficient for labeling surface expressed proteins in 96-well format.

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Description

The Incucyte® Mouse IgG1 FabFluor-488 Antibody Labeling Reagent enables rapid and efficient labeling of Fc-containing antibodies with a green fluorophore for live-cell immunocytochemistry applications. When combined with a background suppressor, the labeled antibody complex allows accurate detection and visualization of surface-expressed antigens directly in living cells. Designed specifically for use with Incucyte® Live-Cell Analysis Systems, this reagent supports a simple no-wash, mix-and-read workflow that minimizes handling and preserves cell integrity. This enables real-time, kinetic monitoring of protein expression and distribution without disrupting normal cell conditions. By streamlining antibody labeling and assay setup, the reagent facilitates high-throughput analysis in microplate formats while delivering reproducible, quantitative insights into dynamic cellular processes.

Key Features / Benefits

  • Rapid labeling of Fc-containing antibodies with green fluorophore
  • No-wash, mix-and-read immunocytochemistry workflow
  • Enables real-time, kinetic measurement of surface protein expression
  • Minimizes cell handling to preserve cell health and integrity
  • Designed for seamless use with Incucyte® systems
  • Suitable for 96-well plate assays

Applications

  • Live-cell immunocytochemistry
  • Surface protein expression analysis
  • Receptor binding and trafficking studies
  • Antibody screening and validation
  • High-throughput cell-based assays

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